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Cortisol, ACTH Stimulation

CPT: 82533(x2)
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  • Compound F (Two Specimens)
  • Cortisol (Two Specimens)
  • Cosyntropin Stimulation
  • Hydrocortisone (Two Specimens)

Special Instructions

Draw blood for baseline cortisol. Inject cosyntropin 250 μg IM or IV (if IV, dilute cosyntropin in 2-5 mL of sterile saline and inject over two minutes). Draw blood for stimulated cortisol 60 minutes after. See the online Endocrine Appendix: ACTH Stimulation for expected values for various steroids at baseline and after ACTH stimulation.

This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.

Expected Turnaround Time

1 - 2 days

Related Information

Related Documents

For more information, please view the literature below.

Adrenal Steroid Response to ACTH: Pediatrics

Specimen Requirements




0.8 mL (each specimen)

Minimum Volume

0.3 mL


Red-top tube or gel-barrier tube


If tube other than a gel-barrier tube is used, transfer separated serum to a plastic transport tube. Note: Label each tube with the patient's name, collection time, and date. Submit specimens simultaneously on the same test request form.

Storage Instructions

Room temperature.

Stability Requirements



Room temperature

14 days


14 days


14 days

Freeze/thaw cycles

Stable x3

Causes for Rejection

Plasma specimen

Test Details


This test may involve significant risk and should be performed only by qualified personnel who are familiar with the test and who have taken adequate precautions to protect the safety of the patient. Every effort has been made to ensure accuracy in these recommendations, but clinicians must use their judgment and refer to specific pharmaceutical resources to determine appropriate drug dosages for their patients.


Electrochemiluminescence immunoassay (ECLIA)


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
140761 Cortisol, ACTH Stimulation 140792 Cortisol Baseline ug/dL 43215-3
140761 Cortisol, ACTH Stimulation 140793 Cortisol Stimulated ug/dL 34476-2

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