Mpox (Orthopoxvirus), DNA, PCR

CPT: 87593
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  • Monkeypox Virus PCR

Expected Turnaround Time

3 - 4 days

Related Documents

For more information, please view the literature below.

Monkeypox Specimen Collection Guide

Specimen Requirements


Lesion swab


One swab in UTM or VTM

Minimum Volume

One swab in UTM or VTM


Universal Transport Media (UTM) or Viral Transport Media (VTM)


Vigorously swab or brush the base of the lesion with a sterile dry polyester, rayon or Dacron swab. Insert the swab into the tube containing UTM or VTM. Carefully break the swab at the scoreline and tightly close the sample. Some UTM kits may contain two swabs; however, only one swab needs to be collected and submitted for testing. If multiple lesions with differing appearances are present, consider submitting an additional UTM/VTM collection, as described above, for each lesion.

Storage Instructions


Stability Requirements




7 days (stability provided by manufacturer or literature reference)


30 days (stability provided by manufacturer or literature reference)

Causes for Rejection

Improperly submitted specimens, including grossly leaking samples; swabs submitted in non-validated transport media, including 0.9% saline or liquid Amies (E-Swabs); swab specimens submitted in aerobic or anaerobic transport devices

Test Details


Vesicular specimens collected from persons infected with a non-variola Orthopoxvirus (such as vaccinia, monkeypox, or cowpox) are expected to produce a positive result with this assay. Although this assay does not differentiate vaccinia or monkeypox virus from cowpox, camelpox, ectomelia or gerbilpox virus, a positive result with this assay in the United States is most likely due to monkeypox virus or vaccinia virus; however, potential exposure to other Orthopoxviruses should be considered.


A false negative result may occur if a sample is improperly collected, transported or handled. False negative results may occur if the quantity of viral DNA present in the sample is below the limit of detection for the assay. An inconclusive result may be returned if the lesion is not adequately sampled.


Polymerase chain reaction (PCR)

Additional Information

If monkeypox is suspected, do not submit specimens for any Viral Cultures. Testing for HSV and VZV by molecular methods is available. See Herpes Simplex Virus (HSV) and Varicella Zoster Virus (VZV) Profile (Lesions), PCR [139367] for further information.


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
140230 Monkeypox (Orthopoxvirus), PCR 100434-0 140231 Orthopoxvirus DNA 100434-0

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