Human Immunodeficiency Virus 1 & 2 (HIV-1/HIV-2), Qualitative, RNA

CPT: 87535; 87538
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Expected Turnaround Time

2 - 5 days


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Specimen Requirements


Specimen

Plasma or serum


Volume

1 mL


Minimum Volume

650 µL


Container

Lavender-top (EDTA) tube, BD SST tube, BD Vacutainer PPT plasma preparation tube


Collection

Collect specimen in lavender-top (EDTA), gel-barrier (SST tube), or a PPT plasma preparation tube. Whole blood specimens stored at room temperature should be centrifuged within 24 hours of collection. Transfer serum or plasma to a polypropylene screw-cap tube for shipment.


Storage Instructions

Frozen. Sample may be stored for 24 hours at room temperature. It is recommended that serum or plasma be refrigerated or frozen for shipment.


Stability Requirements

Temperature

Period

Room temperature

24 hours

Refrigerated

7 days

Frozen

6 weeks

Freeze/thaw cycles

Stable x3

Note: Stability provided by manufacturer or literature reference.


Causes for Rejection

Specimen grossly hemolyzed or received outside of specimen and/or storage requirements


Test Details


Use

This test is intended for use as an aid in the diagnosis of HIV-1 or HIV-2 infection, including acute or primary infection. Presence of HIV-1 or HIV-2 RNA in the plasma of patients without antibodies to HIV-1 or HIV-2 is indicative of acute or primary HIV-1 or HIV-2 infection. This assay may also be used as an additional test, when it is reactive, to confirm HIV-1 infection in an individual whose specimen is repeatedly reactive for HIV-1 and/or HIV-2 antibodies. This assay is not intended for use in donor testing.


Methodology

RT-PCR


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
139825 HIV-1/HIV-2 Qualitative RNA 96557-4 139826 HIV-1 RNA 25835-0
139825 HIV-1/HIV-2 Qualitative RNA 96557-4 139827 HIV-2 RNA 69353-1

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