Plasma (preferred) or serum (acceptable)
Lavender-top (EDTA) tube (preferred) or gel-barrier tube
For plasma, draw blood into an EDTA tube and gently invert the tube 8 to 10 times to mix the anticoagulant. Centrifuge the tube, remove the stopper and draw off approximately 2/3 of the upper plasma layer into a labeled transfer tube using a transfer pipet bulb. Note: This ensures the buffy coat of white cells and red cells remain undisturbed. Plasma must be separated from cells within 45 minutes of venipuncture. Send plasma in a plastic transfer tube.
Overnight fasting is preferred.
Specimen other than EDTA plasma or serum; improper labeling; specimen not stored properly; specimen older than stability limits; hemolysis; lipemia
For the in vitro quantitative measurement of oxidized low density lipoproteins (oxidized LDL) in human serum or plasma.
Measurement of oxidized LDL (oxLDL) has been incorporated into clinical practice in the diagnosis and treatment of lipid disorders (such as diabetes mellitus), atherosclerosis, and various liver and renal diseases, especially as it pertains to the evaluation of oxidative stress. Oxidized LDL-particles are considered to be an important driving factor in the pathophysiology of atherosclerosis and oxLDL measurement has been used to test the efficacy of CVD drugs (eg, statins) to reduce oxidative stress.9
Lipemic or hemolytic samples may give erroneous results and should not be used for analysis.
Enzyme-linked immunoassay (ELISA)
The oxidative conversion of low density lipoproteins (LDL) to oxidized low density lipoproteins (oxidized LDL) is now considered to be a key event in the biological process that initiates and accelerates the development of the early atherosclerotic lesion, the fatty streak.1-5
Experimental studies have shown that native LDL becomes atherogenic when it is converted to oxidized LDL, and that oxidized LDL is more atherogenic than native LDL.1-5 Oxidized LDL is found in monocyte-derived macrophages in atherosclerotic lesions, but not in normal arteries.6 The uptake of LDL into macrophages does not occur by way of the classic Brown/Goldstein LDL receptor.7 Numerous studies1-5,8 have established that LDL, the major carrier of blood cholesterol, must first be converted to oxidized LDL so that it can be recognized by "scavenger" or "oxidized LDL receptors" on monocyte-derived macrophages. The binding of oxidized LDL to macrophages is a necessary step by which oxidized LDL induces cholesterol accumulation in macrophages, thus transforming the macrophages into lipid-laden foam cells.8
|Order Code||Order Code Name||Order Loinc||Result Code||Result Code Name||UofM||Result LOINC|
|123023||Oxidized LDL||90364-1||123024||Oxidized LDL||ng/mL||90364-1|
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