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Protein Electrophoresis, Serum and Quantitative Free κ and λ Light Chains (FLC) Plus Ratio, Serum

CPT: 83883(x2); 84155; 84165
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  • Electrophoresis, Serum
  • FLC, Serum
  • Free Light Chains, Quantitative, Serum
  • Kappa Free Light Chains, Quantitative, Serum
  • Kappa/Lambda Free Light Chains, Quantitative, Serum
  • Kappa:Lambda Free Light Chains Ratio, Quantitative, Serum
  • Lambda Free Light Chains, Quantitative, Serum
  • Light Chains, Free κ and Free λ, Quantitative, Serum

Test Includes

Serum protein electrophoresis with relative percentages and quantitation of albumin; α1-, α2-, β-, and γ-globulin fractions; graph of fractions; quantitation of total protein and M-spike if present; quantitation of serum free κ and λ light chains plus κ:λ ratio

Expected Turnaround Time

2 - 5 days

Specimen Requirements




2.5 mL

Minimum Volume

1.2 mL


Red-top tube or gel-barrier tube


Sample should be allowed to clot and the serum separated as soon as possible to prevent hemolysis.

Storage Instructions

PE: Stable when covered at room temperature 25°C for four days; stable refrigerated at 2°C to 8°C for two weeks; stable frozen at -20°C for six months.

FLC: Refrigerate; samples are stable for 21 days at 2°C to 8°C. For longer storage, freeze at -20°C.

Causes for Rejection

Lipemia; hemolysis; plasma specimen

Test Details


Detect and identify monoclonal immunoglobulin gammopathies


Protein electrophoresis: agarose media; free κ and λ light chains: turbidimetric quantitation

Additional Information

Elevated serum levels of monoclonal FLC are associated with malignant plasma cell proliferation (eg, multiple myeloma), primary amyloidosis, and light chain deposition disease. Raised serum levels of polyclonal FLC may be associated with autoimmune diseases such as systemic lupus erythematosus.


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
121210 PE and FLC, Serum 001073 Protein, Total g/dL 2885-2
121210 PE and FLC, Serum 001488 Albumin g/dL 2862-1
121210 PE and FLC, Serum 001489 Alpha-1-Globulin g/dL 2865-4
121210 PE and FLC, Serum 001490 Alpha-2-Globulin g/dL 2868-8
121210 PE and FLC, Serum 001491 Beta Globulin g/dL 2871-2
121210 PE and FLC, Serum 001492 Gamma Globulin g/dL 2874-6
121210 PE and FLC, Serum 001493 M-Spike g/dL 33358-3
121210 PE and FLC, Serum 011052 Globulin, Total g/dL 10834-0
121210 PE and FLC, Serum 011149 A/G Ratio 1759-0
121210 PE and FLC, Serum 011150 Please note: 77202-0
121210 PE and FLC, Serum 001498 PDF 11502-2
121210 PE and FLC, Serum 121138 Free Kappa Lt Chains,S mg/L 36916-5
121210 PE and FLC, Serum 121139 Free Lambda Lt Chains,S mg/L 33944-0
121210 PE and FLC, Serum 121141 Kappa/Lambda Ratio,S 48378-4

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