Thrombotic Risk Profile, Acquired

CPT: 85613; 85705; 85732; 86146(x2); 86147(x2)
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Synonyms

  • Antiphospholipid Syndrome Profile

Test Includes

Anticardiolipin antibodies, IgG and IgM; β2-glycoprotein 1 antibodies, IgG and IgM; dilute prothrombin time profile; lupus anticoagulant with reflex


Expected Turnaround Time

3 - 4 days



Related Documents

For more information, please view the literature below.

Procedures for Hemostasis and Thrombosis: A Clinical Test Compendium


Specimen Requirements


Specimen

Serum and plasma, frozen


Volume

1 mL serum and 1 mL frozen sodium citrate plasma


Container

Red-top tube or gel-barrier tube and blue-top (sodium citrate) tubes


Collection

Citrated plasma samples should be collected by double centrifugation. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.1 Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio.2,3 The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples, except when using a winged blood collection device (ie, "butterfly"), in which case a discard tube should be used.4,5 When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tubes. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge for 10 minutes and (using a plastic transfer pipette) carefully remove two-thirds of the plasma without disturbing the cells. Deliver to a plastic transfer tube, cap, and recentrifuge for 10 minutes. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a LabCorp PP transpak frozen purple tube with screw cap (LabCorp No. 49482). The specimen should be frozen immediately and maintained frozen until tested.

Please print and use the Volume Guide for Coagulation Testing to ensure proper draw volume.


Storage Instructions

Refrigerate serum. Freeze plasma.


Test Details


Use

Detect lupus anticoagulants and antiphospholipid antibodies


Methodology

See individual tests.


Footnotes

1. Adcock DM, Kressin DC, Marlar RA. Effect of 3.2% vs 3.8% sodium citrate concentration on routine coagulation testing. Am J Clin Pathol. 1997; 107(1):105-110. 8980376
2. Reneke J, Etzell J, Leslie S, et al. Prolonged prothrombin time and activated partial thromboplastin time due to underfilled specimen tubes with 109 mmol/L (3.2%) citrate anticoagulant. Am J Clin Pathol. 1998; 109(6):754-757. 9620035
3. National Committee for Clinical Laboratory Standardization. Collection, Transport, and Processing of Blood Specimens for Coagulation Testing and General Performance of Coagulation Assays; Approved Guideline. 5th ed. Villanova, Pa: NCCLS; 2008. Document H21-A5:28(5).
4. Gottfried EL, Adachi MM. Prothrombin time and activated partial thromboplastin time can be performed on the first tube. Am J Clin Pathol. 1997; 107(6):681-683.9169665
5. McGlasson DL, More L, Best HA, et al. Drawing specimens for coagulation testing: Is a second tube necessary? Clin Lab Sci. 1999; 12(3):137-139. 10539100

References

Alving BM. The antiphospholipid syndrome: Clinical presentation, diagnosis, and patient management. In: Kitchens CS, Alving BM, Kessler CM, eds Consultative Hemostasis and Thrombosis. Philadelphia, Pa: WB Saunders Co; 2002:181-196.
Schafer AI, Levine MN, Konkle BA, et al. Thrombotic disorders: Diagnosis and treatment. Hematology (Am Soc Hematol Educ Program). 2003; 520-539. 14633797
Triplett DA. Thrombophilia. In: McClatchey KD, ed. Clinical Laboratory Medicine. 2nd ed. Philadelphia, Pa: Lippincott Williams and Wilkins;2002:1033-1049.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
117024 Thrombotic Risk, Acquired 005201 Dilute Prothrombin Time(dPT) sec 75508-2
117024 Thrombotic Risk, Acquired 005203 dPT Confirm Ratio Ratio 75884-7
117024 Thrombotic Risk, Acquired 161812 Anticardiolipin Ab,IgG,Qn GPL U/mL 3181-5
117024 Thrombotic Risk, Acquired 161830 Anticardiolipin Ab,IgM,Qn MPL U/mL 3182-3
117024 Thrombotic Risk, Acquired 163881 Beta-2 Glycoprotein I Ab, IgG GPI IgG units 16135-6
117024 Thrombotic Risk, Acquired 163907 Beta-2 Glycoprotein I Ab, IgM GPI IgM units 16136-4
117024 Thrombotic Risk, Acquired 117003 PTT-LA sec 34571-0
117024 Thrombotic Risk, Acquired 117891 dRVVT sec 6303-2
117024 Thrombotic Risk, Acquired 117893 Lupus Reflex Interpretation 75514-0
Reflex Table for PTT-LA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 117040 PTT-LA Mix 117005 PTT-LA Mix sec 75510-8
Reflex 2 117020 117842 Hexagonal Phase Phospholipid sec 3282-1
Reflex Table for PTT-LA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 117040 PTT-LA Mix 117005 PTT-LA Mix sec 75510-8
Reflex 2 117035 117036 PTT-LA Incub Mix sec 50760-8
Reflex 3 117020 Hexagonal Phase Phospholipid 117842 Hexagonal Phase Phospholipid sec 3282-1

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