Amylase, Body Fluid

CPT: 82150
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  • Cyst Fluid Amylase

Special Instructions

State source of fluid on the request form (eg, pleural, peritoneal, pericardial, synovial, cyst).

Expected Turnaround Time

1 - 2 days

Related Documents

Specimen Requirements


Body fluid (eg, ascitic fluid, pleural fluid, etc)


1 mL

Minimum Volume

0.3 mL (Note: This volume does not allow for repeat testing.)


Clean container, no preservative


Aspirate fluid and transfer to a clean transport tube ASAP.

Storage Instructions

Maintain specimen at room temperature.

Stability Requirements



Room temperature

14 days


14 days


14 days

Freeze/thaw cycles

Stable x3

Causes for Rejection

Improper labeling

Test Details


Pancreatitis with or without pseudocyst formation or pancreatic pleural fistula is the most common cause of amylase elevation in pleural fluid. Rupture of the esophagus is the second most common group and malignant effusion is the third.1 Other causes include pancreatic ascites and pancreatic duct trauma. Defect in the wall of the gastrointestinal tract (eg, perforated peptic ulcer) will allow pancreatic secretion to enter the peritoneal cavity. Similarly, peritoneal fluid amylase elevations may be found in the presence of necrotic bowel. Peritoneal fluid, containing such amylase, can find its way into a pleural space.


In collection of ascitic fluid, the localization of the catheter is likely to affect the chemistry result.2 Oxalate or citrate depress results. Lipemic sample may contain inhibitors which falsely depress results. Benign ovarian cyst fluids may have significant amylase activity.



Reference Interval

• Peritoneal: 88−109 U/L

• Pleural:

− Transudate: Not established

− Exudate: Not established

• Synovial: Not established

The method performance specifications have not been established for this test in body fluid. The test result should be integrated into the clinical context for interpretation.

Additional Information

Most patients with pancreatic ascites have high peritoneal fluid amylase as well as amylase elevations in serum and urine. Pancreatitis may present with pleural effusion. Of 34 patients who had high amylase in pleural fluid associated with neoplasms, 18 had carcinoma of lung. Other tumors were gynecologic, gastrointestinal, lymphoma, breast and malignancy of unknown origin.1


1. Kramer MR, Saldana MJ, Cepero RJ, Pitchenik AE. High amylase levels in neoplasm-related pleural effusion. Ann Intern Med. 1989 Apr 1; 110(7):567-569. 2466431
2. Robert JH, Meyer P, Rohner A. Can serum and peritoneal amylase and lipase determinations help in the early prognosis of acute pancreatitis? Ann Surg. 1986 Feb; 203(2):163-168. 2418796


Henry RJ, Chiamori N. Study of the saccharogenic method for the determination of serum and urine amylase. Clin Chem. 1960; 6(5):434-452.


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
088062 Amylase, Body Fluid 1795-4 088062 Amylase, Body Fluid U/L 1795-4

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