Rotavirus, Direct Detection Immunoassay

CPT: 87425
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Expected Turnaround Time

1 - 4 days

Related Documents

For more information, please view the literature below.

Microbiology Specimen Collection and Transport Guide

Specimen Requirements




2 mL liquid stool or 1 g semiformed stool


Empty stool transport vial or screw-cap container, sterile no additives


Collect as soon as possible after onset of disease, preferably three to five days after onset. Collection from diapered children can be facilitated by using a disposable diaper “inside out” or by using a “Saran wrap” diaper to capture the next stool specimen.

Storage Instructions

Refrigerate at 2°C to 8°C immediately after collection.

Causes for Rejection

Specimen received in grossly leaking transport container; dry specimen; specimen submitted in fixative or additive; bacterial swab specimen; diapers; unlabeled specimen or name discrepancy between specimen and request label; specimen transported at room temperature; other inappropriate specimen transport devices; specimen received after prolonged delay (usually more than 72 hours); specimen in transport media or preservative

Test Details


Detect rotavirus in stools of patients suspected of having viral gastroenteritis


Quality of specimens cannot be evaluated, and specimens are collected randomly.


Immunoassay (IA)

Reference Interval


Additional Information

Rotavirus is an extremely common cause of pediatric gastroenteritis. The illness is most common in winter, is highly contagious, involves five to eight days of diarrhea, and is rarely fatal.1 Patients should also be evaluated for possible bacterial gastroenteritis. Outbreaks are seen among children in daycare centers.


1. Christensen ML, Howard C. Viruses causing gastroenteritis. In: Balows A, Hausler WJ, et al, eds. Manual of Clinical Microbiology. 5th ed. Washington, DC: ASM Press;1991:950-958.


Christensen ML. Human viral gastroenteritis. Clin Microbiol Rev. 1989 Jan; 2(1):51-89. 2644024
Gray LD. Novel viruses associated with gastroenteritis. Clin Microbiol Newslet. 1991; 13(18):137-144.


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
006866 Rotavirus Ag, EIA 5880-0 006866 Rotavirus Ag, EIA 5880-0

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