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CPT:
85025
Special Instructions
Note special reflexing: If Hemoglobin (Hb) [005041] is low, test will reflex to Ferritin [004598]. If Ferritin is normal, test will reflex to Iron and Total Iron-binding Capacity (TIBC) [001321]. Depending on results of Iron and TIBC, test may reflex to Soluble Transferrin Receptor [143305], all at an additional charge or CPT code.
Expected Turnaround Time
Within 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Documents
Specimen Requirements
Specimen
Serum and whole blood
Volume
4 mL serum and 4 mL whole blood (fill tube to capacity)
Minimum Volume
Serum: 2 mL
Whole blood: 0.5 mL or 500 µL for pediatric microtainer capillary tubes; venous tube collection: fill tube to capacity
Container
Gel-barrier tube and lavender-top (EDTA) tube
Collection
Serum: Separate serum from cells within 45 minutes of collection.
Lavender-top: Invert tube 8 to 10 times immediately after tube is filled at the time of collection.
Storage Instructions
Maintain one serum and lavender-top tube at room temperature. Refrigerate one serum transport tube.
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | Serum: 14 days Whole blood: 1 day |
Refrigerated | Serum: 14 days Whole blood: 3 days |
Frozen | Serum: 14 days Whole blood: Unstable |
Causes for Rejection
Serum: Hemolysis; specimen clotted; improper labeling; EDTA, yellow-top tube, citrate or oxalate plasma specimen
Whole blood: Hemolysis; clotted specimen; specimen drawn in any anticoagulant other than EDTA; specimen diluted or contaminated with IV fluid; tube not filled with minimum volume; improper labeling; transfer tubes with whole blood; specimen received with plasma removed (plasma is used for other testing)
Test Details
Use
This test is used to determine iron deficiency anemia.
Methodology
See individual test components.
References
Baillie FJ, Morrison AE, Fergus I. Soluble transferrin receptor: a discriminating assay for iron deficiency. Clin Lab Haematol. 2003 Dec;25(6):353-357.14641138
Camaschella C. Iron deficiency: new insights into diagnosis and treatment. Hematology Am Soc Hematol Educ Program. 2015;2015:8-13.26637694
Cappellini MD, Musallam KM, Taher AT. Iron deficiency anaemia revisited. J Intern Med. 2020 Feb;287(2):153-170.31665543
Daru J, Allotey J, Pea-Rosas JP, Khan KS. Serum ferritin thresholds for the diagnosis of iron deficiency in pregnancy: a systematic review. Transfus Med. 2017 Jun;27(3):167-174.28425182
Farrington DK, Sang Y, Grams ME, et al. Anemia Prevalence, Type, and Associated Risks in a Cohort of 5.0 Million Insured Patients in the United States by Level of Kidney Function. Am J Kidney Dis. 2023 Feb;81(2):201-209.e1. Epub 22 Sep 28.36181996
Kumar A, Sharma E, Marley A, Samaan MA, Brookes MJ. Iron deficiency anaemia: pathophysiology, assessment practical management. BMJ Open Gastroenterol. 2022 Jan;9(1):e000759.34996762
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