Iron Deficiency Anemia Cascade With Complete Blood Count (CBC) With Differential

CPT: To be determined. Updates will be made when available.

Special Instructions

Note special reflexing: If Hemoglobin (Hb) [005041] is low, test will reflex to Ferritin [004598]. If Ferritin is normal, test will reflex to Iron and Total Iron-binding Capacity (TIBC) [001321]. Depending on results of Iron and TIBC, test may reflex to Soluble Transferrin Receptor [143305], all at an additional charge or CPT code.


Expected Turnaround Time

Within 5 days


Related Documents

For more information, please view the literature below.

Iron Deficiency Anemia Cascade White Paper


    Specimen Requirements


    Specimen

    Serum and whole blood


    Volume

    4 mL serum and 4 mL whole blood (fill tube to capacity)


    Minimum Volume

    Serum: 2 mL

    Whole blood: 0.5 mL or 500 L for pediatric microtainer capillary tubes; venous tube collection: fill tube to capacity


    Container

    Gel-barrier tube and lavender-top (EDTA) tube


    Collection

    Serum: Separate serum from cells within 45 minutes of collection.

    Lavender-top: Invert tube 8 to 10 times immediately after tube is filled at the time of collection.


    Storage Instructions

    Maintain one serum and lavender-top tube at room temperature. Refrigerate one serum transport tube.


    Stability Requirements

    Temperature

    Period

    Room temperature

    Serum: 14 days

    Whole blood: 1 day

    Refrigerated

    Serum: 14 days

    Whole blood: 3 days

    Frozen

    Serum: 14 days

    Whole blood: Unstable


    Causes for Rejection

    Serum: Hemolysis; specimen clotted; improper labeling; EDTA, yellow-top tube, citrate or oxalate plasma specimen

    Whole blood: Hemolysis; clotted specimen; specimen drawn in any anticoagulant other than EDTA; specimen diluted or contaminated with IV fluid; tube not filled with minimum volume; improper labeling; transfer tubes with whole blood; specimen received with plasma removed (plasma is used for other testing)


    Test Details


    Use

    This test is used to determine iron deficiency anemia.


    Methodology

    See individual test components.


    References

    Baillie FJ, Morrison AE, Fergus I. Soluble transferrin receptor: a discriminating assay for iron deficiency. Clin Lab Haematol. 2003 Dec;25(6):353-357.14641138
    Camaschella C. Iron deficiency: new insights into diagnosis and treatment. Hematology Am Soc Hematol Educ Program. 2015;2015:8-13.26637694
    Cappellini MD, Musallam KM, Taher AT. Iron deficiency anaemia revisited. J Intern Med. 2020 Feb;287(2):153-170.31665543
    Daru J, Allotey J, Pea-Rosas JP, Khan KS. Serum ferritin thresholds for the diagnosis of iron deficiency in pregnancy: a systematic review. Transfus Med. 2017 Jun;27(3):167-174.28425182
    Farrington DK, Sang Y, Grams ME, et al. Anemia Prevalence, Type, and Associated Risks in a Cohort of 5.0 Million Insured Patients in the United States by Level of Kidney Function. Am J Kidney Dis. 2023 Feb;81(2):201-209.e1. Epub 22 Sep 28.36181996
    Kumar A, Sharma E, Marley A, Samaan MA, Brookes MJ. Iron deficiency anaemia: pathophysiology, assessment practical management. BMJ Open Gastroenterol. 2022 Jan;9(1):e000759.34996762

    For Providers

    Please login to order a test

    Order a Test

    © 2021 Laboratory Corporation of America® Holdings and Lexi-Comp Inc. All Rights Reserved.

    CPT Statement/Profile Statement

    The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf