Iron Deficiency Anemia Cascade With Complete Blood Count (CBC) With Differential

CPT: To be determined. Updates will be made when available.

Special Instructions

Note special reflexing: If Hemoglobin (Hb) [005041] is low, test will reflex to Ferritin [004598]. If Ferritin is normal, test will reflex to Iron and Total Iron-binding Capacity (TIBC) [001321]. Depending on results of Iron and TIBC, test may reflex to Soluble Transferrin Receptor [143305], all at an additional charge or CPT code.

Expected Turnaround Time

Within 5 days

Related Documents

For more information, please view the literature below.

Iron Deficiency Anemia Cascade White Paper

    Specimen Requirements


    Serum and whole blood


    4 mL serum and 4 mL whole blood (fill tube to capacity)

    Minimum Volume

    Serum: 2 mL

    Whole blood: 0.5 mL or 500 L for pediatric microtainer capillary tubes; venous tube collection: fill tube to capacity


    Gel-barrier tube and lavender-top (EDTA) tube


    Serum: Separate serum from cells within 45 minutes of collection.

    Lavender-top: Invert tube 8 to 10 times immediately after tube is filled at the time of collection.

    Storage Instructions

    Maintain one serum and lavender-top tube at room temperature. Refrigerate one serum transport tube.

    Stability Requirements



    Room temperature

    Serum: 14 days

    Whole blood: 1 day


    Serum: 14 days

    Whole blood: 3 days


    Serum: 14 days

    Whole blood: Unstable

    Causes for Rejection

    Serum: Hemolysis; specimen clotted; improper labeling; EDTA, yellow-top tube, citrate or oxalate plasma specimen

    Whole blood: Hemolysis; clotted specimen; specimen drawn in any anticoagulant other than EDTA; specimen diluted or contaminated with IV fluid; tube not filled with minimum volume; improper labeling; transfer tubes with whole blood; specimen received with plasma removed (plasma is used for other testing)

    Test Details


    This test is used to determine iron deficiency anemia.


    See individual test components.


    Baillie FJ, Morrison AE, Fergus I. Soluble transferrin receptor: a discriminating assay for iron deficiency. Clin Lab Haematol. 2003 Dec;25(6):353-357.14641138
    Camaschella C. Iron deficiency: new insights into diagnosis and treatment. Hematology Am Soc Hematol Educ Program. 2015;2015:8-13.26637694
    Cappellini MD, Musallam KM, Taher AT. Iron deficiency anaemia revisited. J Intern Med. 2020 Feb;287(2):153-170.31665543
    Daru J, Allotey J, Pea-Rosas JP, Khan KS. Serum ferritin thresholds for the diagnosis of iron deficiency in pregnancy: a systematic review. Transfus Med. 2017 Jun;27(3):167-174.28425182
    Farrington DK, Sang Y, Grams ME, et al. Anemia Prevalence, Type, and Associated Risks in a Cohort of 5.0 Million Insured Patients in the United States by Level of Kidney Function. Am J Kidney Dis. 2023 Feb;81(2):201-209.e1. Epub 22 Sep 28.36181996
    Kumar A, Sharma E, Marley A, Samaan MA, Brookes MJ. Iron deficiency anaemia: pathophysiology, assessment practical management. BMJ Open Gastroenterol. 2022 Jan;9(1):e000759.34996762

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