004564: Estrone

Test Details

Methodology

Enzyme immunoassay (EIA)

Result Turnaround Time

4 - 5 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Use

This test is used to evaluate postmenopausal vaginal bleeding due to peripheral conversion of androgenic steroids. Increased estrone levels may be associated with increased levels of circulating androgens and their subsequent peripheral conversion.

Special Instructions

Nonpregnant patients only.

Custom Additional Information

Estrone (E1) is a steroid, a weak estrogen, and a minor female sex hormone.^3,4 Estrone is one of three major endogenous estrogens, the others being estradiol and estriol.^3,4 Like the other estrogens, estrone is synthesized from cholesterol and secreted mainly from the gonads, though they can also be formed from adrenal androgens in adipose tissue. Estrone is primarily derived from metabolism of androstenedione in peripheral tissues, especially adipose tissues. Individuals with obesity have increased conversion of androstenedione to estrone, leading to higher concentrations. Estrone can be converted into estradiol and serves mainly as a precursor or metabolic intermediate of estradiol. In addition, an increase in the ratio of estrone to estradiol may be useful in assessing menopause in women.

Specimen Requirements

Specimen

Serum from a serum separator tube

Volume

0.6 mL

Minimum Volume

0.4 mL (Note: This volume does not allow for repeat testing.)

Container

Gel-barrier serum separator tube; red top tubes (without gel barrier) should not be used

Stability Requirements

Temperature	Period
Room temperature	14 days
Refrigerated	14 days
Frozen	14 days
Freeze/thaw cycles	Stable x3

Reference Range

See table.

Age (y)	Male (pg/mL)^1,2	Female (pg/mL)^1,2
Tanner Stage I	5−17	4−29
Tanner Stage II	10−25	10−33
Tanner Stage III	15−25	15−43
Tanner Stage IV	15−45	16−77
Tanner Stage V	20−45	29−105
Adults	0−174	Adult (Premenopausal): 27−231
		Menstrual Cycle (1-10 days): 19−149
		Menstrual Cycle (11-20 days): 32−176
		Menstrual Cycle (21-30 days): 37−200
		Adult (Postmenopausal): 0−125

Storage Instructions

Room temperature

Causes for Rejection

Sample collected with any tube other than a gel barrier (serum separator) tube; hemolysis

References

Gruber CJ, Tschugguel W, Schneeberger C, Huber JC. Production and actions ofestrogens. N Engl J Med. 2002 Jan 31;346(5):340-352.11821512

Kronenberg H, Williams HR. The physiology and pathology of the female reporductive axis. In: Williams Textbook of Endocrinology. 11th ed. Philadelphia, Pa: Saunders/Elsevier; 2008:541-614.

Nelson LR, Bulun SE. Estrogen production and action. J Am Acad Dermatol. 2001 Sep;45(3 Suppl):S116-24.11511861

Footnotes

1. Tietz NW, ed. Clinical Guide to Laboratory Tests. 4th ed. Philadelphia, Pa: WB Saunders Co; 2006:378-379.

2. Estrone RIA DSL8700-FOC-02 [package insert]. Beckman Coulter/ImmunoTech; 2018.

3. Coelingh Bennink HJT. Are all estrogens the same? Maturitas. 2004 Apr 15;47(4):269-275.15063479

4. Kuhl H. Pharmacology of estrogens and progestogens: influence of different routes of administration. Climacteric. 2005 Aug;8 Suppl 1:3-63.16112947

LOINC® Map

Order Code	Order Code Name	Order Loinc	Result Code	Result Code Name	UofM	Result LOINC
004564	Estrone, Serum	2258-2	004564	Estrone, Serum	pg/mL	2258-2
Order Code	004564
Order Code Name	Estrone, Serum
Order Loinc	2258-2
Result Code	004564
Result Code Name	Estrone, Serum
UofM	pg/mL
Result LOINC	2258-2

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