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Amylase, Urine

CPT: 82150
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Test Includes

Amylase on random or timed urine collections

Special Instructions

The test request form must state date and time collection started, date and time collection finished, and urine volume.

Expected Turnaround Time

1 - 3 days

Related Information

Related Documents

Specimen Requirements


Urine (timed)


10 mL aliquot

Minimum Volume

0.5 mL aliquot (Note: This volume does not allow for repeat testing.)


Plastic urine container without preservative


Collect timed specimen. Instruct the patient to void at the beginning of the collection period and discard the specimen. Collect all urine including the final specimen voided at the end of collection period.

Storage Instructions

Room temperature

Stability Requirements



Room temperature

14 days


14 days


3 days

Freeze/thaw cycles

Stable x3

Causes for Rejection

Presence of acid preservative (pH <3); improper labeling

Test Details


Work-up for abdominal pain, epigastric tenderness, nausea, and vomiting. An enzyme with molecular weight of 45 to 55 kilodaltons, urinary amylase is used in the differential diagnosis of pancreatitis. It is very useful in diagnosis of pseudocyst of the pancreas, where the urine amylase may remain elevated for weeks after the serum amylase has returned to normal, after a bout of acute pancreatitis.



Reference Interval


Male (U/hr)

Female (U/hr)

0 to 17 y

Not established

Not established

>17 y



Additional Information

Macroamylasemia is characterized by high serum amylase but normal urine amylase. The amylase:creatinine ratio remains useful for the diagnosis of macroamylasemia, but its nonspecificity has otherwise left it with few other applications. In macroamylasemia the clearance is very low.1

Unlike serum amylase, urine amylase levels are normal with renal failure. While serum amylase usually returns to normal within three to five days, without complications urine amylase is increased longer than serum amylase in acute pancreatitis. Two-hour collections are more practical and provide results sooner than longer collections.

The major test for pancreatitis additional to serum and urine amylase is serum lipase. It has good specificity and its laboratory analysis is greatly improved from the 1960s. Also, some patients with pancreatitis have very high triglyceride levels. Immunoreactive trypsin is not available stat and is not widely available at all.


1. Eckfeldt JH, Levitt MD. Diagnostic enzymes for pancreatic disease. Clin Lab Med. 1989 Dec; 9(4):731-743. 2480201


Street HV, Close JR. Determination of amylase activity in biological fluids. Clin Chim Acta. 1956 May-Jun; 1(3):256-268. 13343527


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
003293 Amylase, Urine 003897 Amylase Urine U/L 1799-6
003293 Amylase, Urine 133462 Timing Interval hr 13362-9
003293 Amylase, Urine 010785 Amylase, Urine U/hr 38193-9

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