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- Stool NAAT
- Stool PCR
Adenovirus F 40/41; astrovirus; Campylobacter; Clostridium difficile toxin A/B; Cryptosporidium; Cyclospora cayetanensis; E coli O157; Entamoeba histolytica; enteroaggregative E coli (EAEC); enteropathogenic E coli (EPAC); enterotoxigenic E coli (ETEC) lt/st; Giardia lamblia; norovirus GI/GII; Plesiomonas shigelloides; rotavirus A; Salmonella; Sapovirus; Shiga-like toxin-producing E coli (STEC) stx1/stx2; Shigella/enteroinvasive E coli (EIEC); Vibrio; Vibrio cholerae; Yersinia enterocolitica
Gastrointestinal disease can be caused by many agents, including bacteria, viruses and parasites. Culture for bacteria will not pick up the viruses or parasites. Tests for parasites will not detect the viruses or the bacteria. This GI profile is a multiplexed nucleic acid test intended for the simultaneous qualitative detection and identification of nucleic acids from multiple bacteria, viruses and parasites directly from stool samples in Cary-Blair transport media obtained from individuals with signs and/or symptoms of gastrointestinal infection.
The performance of this test has not been established for patients without signs and symptoms of gastrointestinal illness. Virus, bacteria and parasite nucleic acid may persist in vivo independently of organism viability. Additionally, some organisms may be carried asymptomatically. Detection of organism targets does not imply that the corresponding organisms are infectious or are the causative agents for clinical symptoms. The detection of organism nucleic acid is dependent upon proper sample collection, handling, transportation, storage and preparation. Failure to observe proper procedures in any one of these steps can lead to incorrect results. There is a risk of false-positive and false-negative results caused by improperly collected, transported or and handled specimens.
Polymerase chain reaction (PCR)
Stool placed in a stool culture transport vial (Para-Pak® C&S orange)
Add stool until level in Para-Pak® vial is at fill-line on vial label. Do not over fill.
Para-Pak® orange or sterile container with Cary-Blair preservative liquid medium
If possible, allow patient to urinate before collecting stool specimen so as to avoid contaminating the stool specimen with urine. Catch the stool specimen in a clean, empty wide-mouthed container or place plastic wrap over the opening of the toilet bowl to prevent the stool specimen from falling into the bowl. Do not mix urine or water with the stool specimen. For diaper collected specimens, line the diaper with plastic wrap. Do not submit the diaper. Place small amounts of the stool specimen into the orange-labeled Para-Pak® vial using the spoon affixed to the Para-Pak® vial cap, taking care not to let the volume in the vial exceed the RED fill-line indicated on the vial label. Cap the Para-Pak® vial and shake the vial 10 times to ensure complete distribution of the stool into the preservative. The stool specimen must be placed into the Para-Pak® vial within one hour of the stools production for optimum results. Label the Para-Pak® vial with the patient's name, date of birth, date of collection and time of collection. Be sure the Para-Pak® vial cap is securely in place and that the vial is not leaking.
Refrigerate. Room temperature storage and transport up to four days is acceptable.
Causes for Rejection
Specimen not received in Cary-Blair preservative medium; specimen vial leaking; specimen >96 hours since collection; frozen specimen; rectal swab
Introduce stool into orange Para-Pak® vial in small amounts such that level does not exceed fill-line on label. Cap securely and shake vial to distribute sample into the Cary-Blair preservative.
|Order Code||Order Code Name||Order Loinc||Result Code||Result Code Name||UofM||Result LOINC|
|183480||GI Profile, Stool, PCR||82195-9||183481||Campylobacter||82196-7|
|183480||GI Profile, Stool, PCR||82195-9||183482||C difficile toxin A/B||82197-5|
|183480||GI Profile, Stool, PCR||82195-9||183484||Plesiomonas shigelloides||82198-3|
|183480||GI Profile, Stool, PCR||82195-9||183485||Salmonella||82199-1|
|183480||GI Profile, Stool, PCR||82195-9||183486||Vibrio||82200-7|
|183480||GI Profile, Stool, PCR||82195-9||183487||Vibrio cholerae||82201-5|
|183480||GI Profile, Stool, PCR||82195-9||183488||Yersinia enterocolitica||82202-3|
|183480||GI Profile, Stool, PCR||82195-9||183489||Enteroaggregative E coli||80349-4|
|183480||GI Profile, Stool, PCR||82195-9||183490||Enteropathogenic E coli||80348-6|
|183480||GI Profile, Stool, PCR||82195-9||183492||Enterotoxigenic E coli||80351-0|
|183480||GI Profile, Stool, PCR||82195-9||183493||Shiga-toxin-producing E coli||82203-1|
|183480||GI Profile, Stool, PCR||82195-9||183494||E coli O157||82204-9|
|183480||GI Profile, Stool, PCR||82195-9||183495||Shigella/Enteroinvasive E coli||80350-2|
|183480||GI Profile, Stool, PCR||82195-9||183496||Cryptosporidium||82205-6|
|183480||GI Profile, Stool, PCR||82195-9||183497||Cyclospora cayetanensis||82206-4|
|183480||GI Profile, Stool, PCR||82195-9||183498||Entamoeba histolytica||82207-2|
|183480||GI Profile, Stool, PCR||82195-9||183500||Giardia lamblia||82208-0|
|183480||GI Profile, Stool, PCR||82195-9||183501||Adenovirus F 40/41||82209-8|
|183480||GI Profile, Stool, PCR||82195-9||183502||Astrovirus||82210-6|
|183480||GI Profile, Stool, PCR||82195-9||183503||Norovirus GI/GII||82211-4|
|183480||GI Profile, Stool, PCR||82195-9||183504||Rotavirus A||82212-2|
|183480||GI Profile, Stool, PCR||82195-9||183505||Sapovirus||82213-0|