Tissue Transglutaminase (tTG), IgA

CPT: 86364
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Expected Turnaround Time

2 - 3 days


Related Documents

For more information, please view the literature below.

Celiac Disease Testing Services


Specimen Requirements


Specimen

Serum


Volume

1 mL


Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)


Container

Red-top tube or gel-barrier tube


Collection

Specimen should be free of bacterial contamination, hemolysis, and lipemia.


Storage Instructions

Room temperature


Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3


Causes for Rejection

Hemolysis; lipemia; gross bacterial contamination


Test Details


Use

Detection of antibodies to aid in the diagnosis of gluten-sensitive enteropathy (GSE), such as celiac disease and dermatitis herpetiformis


Limitations

A negative tTG IgA result in an untreated patient does not rule out gluten-sensitive enteropathy. This result can often be explained by selective IgA deficiency, a relatively frequent finding in celiac disease. Results of this assay should be used in conjunction with clinical findings and other serological tests.


Methodology

Enzyme immunoassay (EIA)


Reference Interval

• Negative: 0−3 units/mL

• Weak positive: 4−10 units/mL

• Positive: >10 units/mL


Additional Information

Studies have demonstrated that IgA endomysial antibody tests have >99% specificity for gluten-sensitive enteropathy.1 Recently, the endomysial antigen has been identified as the protein cross-linking enzyme known as tissue transglutaminase (tTG).2


Footnotes

1. Volta U, Molinaro N, De Franchis R, et al. Correlation between IgA antiendomysial antibodies and subtotal villous atrophy in dermatitis herpetiformis. J Clin Gastroenterol. 1992 Jun; 14(4):298-301. 1607605
2. Dieterich W, Ehnis T, Bauer M, et al. Identification of tissue transglutaminase as the autoantigen of celiac disease. Nat Med. 1997 Jul; 3(7):797-801. 9212111

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
164640 t-Transglutaminase (tTG) IgA 31017-7 164643 t-Transglutaminase (tTG) IgA U/mL 31017-7

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