T-Cell Activation Profile, CD8 Subsets

CPT: 86356(x3); 86359; 86360
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Test Details

Test Includes

Percentage and absolute values CD3+, CD3+CD4+, CD3+CD8+, CD3+CD25+, CD8+CD38+, CD8+HLA-DR+, CD4:CD8 ratio; CBC with differential

Use

Expanded profile for AIDS monitoring and prognosis. An increase in activation markers is associated with poor prognosis.

Limitations

This test was developed, and its performance characteristics determined, by LabCorp. It has not been cleared or approved by the US Food and Drug Administration (FDA).

Methodology

Flow cytometry

Reference Interval

Reference intervals are established by the laboratory. See table.

Adult Reference Intervals for Lymphocyte Immunophenotyping

CD

95% Confidence Interval

Percentage

Cells/mm3

Minimum

Maximum

Minimum

Maximum

CD3+

57.5

86.2

622

2402

CD3+CD4+

30.8

58.5

359

1519

CD3+CD8+

12.0

35.5

109

897

CD3+CD25+

4.9

25.9

79

535

T4:T8 ratio

0.92

3.72

CD8+CD38+

0

17.7

0

381

CD8+HLA-DR+

0

4.9

0

117

Specimen Requirements

Specimen

Whole blood

Volume

Fill tube(s) to capacity.

Container

Lavender-top (EDTA) tube and yellow-top (ACD-A) or (ACD-B) tube

Collection

Invert tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimen so it will arrive in the laboratory within 48 hours of collection. Indicate date and time of venipuncture on the tube(s) and on the test request form.

Storage Instructions

Maintain specimen at room temperature.

Stability Requirements

Temperature

Period

Room temperature

2 days

Refrigerated

Unstable

Frozen

Unstable

Freeze/thaw cycles

Unstable

Causes for Rejection

Hemolysis; specimen refrigerated or frozen; clotted specimen; contaminated specimen

Clinical Information

References

Landay AL, Jessop C, Lennette ET, Levy JA. Chronic fatigue syndrome: Clinical condition associated with immune activation. Lancet. 1991 Sep 21; 338(8769):707-712. 1679864
Sadler D, Keren DF. Surface marker assays in immunodeficiency diseases. In: Keren DF, Hanson CA, Hurtubise PE, eds. Flow Cytometry and Clinical Diseases. Chicago, Ill: ASCP Press;1994:chap 9.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
505750 T-Cell Activation, CD8 Subsets 505350 Absolute CD 3 /uL 8122-4
505750 T-Cell Activation, CD8 Subsets 505324 % CD 3 Pos. Lymph. % 8124-0
505750 T-Cell Activation, CD8 Subsets 505351 Absolute CD 4 Helper /uL 24467-3
505750 T-Cell Activation, CD8 Subsets 505326 % CD 4 Pos. Lymph. % 8123-2
505750 T-Cell Activation, CD8 Subsets 505352 Absolute CD 8 (Supp) /uL 14135-8
505750 T-Cell Activation, CD8 Subsets 505328 % CD 8 Pos. Lymph. % 8101-8
505750 T-Cell Activation, CD8 Subsets 505333 CD4/CD8 Ratio 54218-3
505750 T-Cell Activation, CD8 Subsets 505162 Abs.CD8+HLA-DR+Lymph /uL 16107-5
505750 T-Cell Activation, CD8 Subsets 505161 % CD8+HLA-DR+ Lymphs % 13337-1
505750 T-Cell Activation, CD8 Subsets 505157 % CD3+CD25+ Lymphs % 13333-0
505750 T-Cell Activation, CD8 Subsets 505158 Abs.CD3+CD25+ Lymphs /uL 26997-7
505750 T-Cell Activation, CD8 Subsets 505151 % CD8+CD38+ Lymphs % 13344-7
505750 T-Cell Activation, CD8 Subsets 505153 Abs.CD8+CD38+ Lymphs /uL 21374-4
505750 T-Cell Activation, CD8 Subsets 005025 WBC x10E3/uL 6690-2
505750 T-Cell Activation, CD8 Subsets 005033 RBC x10E6/uL 789-8
505750 T-Cell Activation, CD8 Subsets 005041 Hemoglobin g/dL 718-7
505750 T-Cell Activation, CD8 Subsets 005058 Hematocrit % 4544-3
505750 T-Cell Activation, CD8 Subsets 015065 MCV fL 787-2
505750 T-Cell Activation, CD8 Subsets 015073 MCH pg 785-6
505750 T-Cell Activation, CD8 Subsets 015081 MCHC g/dL 786-4
505750 T-Cell Activation, CD8 Subsets 105007 RDW % 788-0
505750 T-Cell Activation, CD8 Subsets 015172 Platelets x10E3/uL 777-3
505750 T-Cell Activation, CD8 Subsets 015107 Neutrophils % 770-8
505750 T-Cell Activation, CD8 Subsets 015123 Lymphs % 736-9
505750 T-Cell Activation, CD8 Subsets 015131 Monocytes % 5905-5
505750 T-Cell Activation, CD8 Subsets 015149 Eos % 713-8
505750 T-Cell Activation, CD8 Subsets 015156 Basos % 706-2
505750 T-Cell Activation, CD8 Subsets 115398 Immature Cells N/A
505750 T-Cell Activation, CD8 Subsets 015909 Neutrophils (Absolute) x10E3/uL 751-8
505750 T-Cell Activation, CD8 Subsets 015917 Lymphs (Absolute) x10E3/uL 731-0
505750 T-Cell Activation, CD8 Subsets 015925 Monocytes(Absolute) x10E3/uL 742-7
505750 T-Cell Activation, CD8 Subsets 015933 Eos (Absolute) x10E3/uL 711-2
505750 T-Cell Activation, CD8 Subsets 015941 Baso (Absolute) x10E3/uL 704-7
505750 T-Cell Activation, CD8 Subsets 015108 Immature Granulocytes % 38518-7
505750 T-Cell Activation, CD8 Subsets 015911 Immature Grans (Abs) x10E3/uL 51584-1
505750 T-Cell Activation, CD8 Subsets 015945 NRBC % 58413-6
505750 T-Cell Activation, CD8 Subsets 015180 Hematology Comments: 18314-5
505750 T-Cell Activation, CD8 Subsets 000000 HIV Confidential N/A
Reflex Table for Immature Granulocytes
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 015111 Please note: 015111 Please note: N/A
Reflex Table for Immature Grans (Abs)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 015111 Please note: 015111 Please note: N/A
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115247 Neutrophils % 23761-0
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115250 Lymphs % 737-7
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115251 Monocytes % 744-3
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115252 Eos % 714-6
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115253 Basos % 707-0
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115398 Immature Cells N/A
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115254 Neutrophils Absolute X10E3/uL 753-4
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115255 Lymphs (Absolute) X10E3/uL 732-8
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115256 Monocytes(Absolute) X10E3/uL 743-5
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115257 Eos (Absolute Value) X10E3/uL 712-0
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115258 Baso(Absolute) X10E3/uL 705-4
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115260 Differential Comment 18723-7
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 005034 RBC Comment 6742-1
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 015171 Platelet Comment 9317-9

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