Reticulocyte Count

CPT: 85045
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Test Includes

Reticulocytes are expressed as a percentage in a total of 1000 RBCs.


Expected Turnaround Time

Within 1 day


Related Documents


Specimen Requirements


Specimen

Whole blood


Volume

Tube fill capacity


Minimum Volume

500 μL for Microtainer™ (capillary) specimens


Container

Lavender-top (EDTA) tube


Collection

Invert tube immediately 8 to 10 times once tube is filled at time of collection.


Storage Instructions

Refrigerate


Stability Requirements

Temperature

Period

Room temperature

1 day

Refrigerated

3 days

Frozen

Unstable

Freeze/thaw cycles

Unstable


Causes for Rejection

Hemolysis; specimen more than 72 hours old; clotted specimen; tube not filled with minimum volume; improper labeling; transfer tubes with whole blood; specimen diluted or contaminated with I.V. fluid; specimen drawn in any anticoagulant other than EDTA; specimen received with plasma removed


Test Details


Use

Evaluate erythropoietic activity which is increased in acute and chronic hemorrhage, and hemolytic anemias; evaluate erythropoietic response to antianemic therapy


Limitations

Caution should be exercised with recently transfused patients (reticulocytes may decrease on a dilutional basis due to transfusion).


Methodology

Automated cell counter


Contraindications

Patients receiving a large number of blood transfusions

Additional Information

Reticulocytes stained with a fluorescent reagent can be differentiated from mature red cells and other cell populations by light scatter, direct measurements, and opacity characteristics when using an automated hematology analyzer equipped with reticulocyte counting technology.


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
005280 Reticulocyte Count 17849-1 005280 Reticulocyte Count % 17849-1

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