Pyruvic Acid, Whole Blood

CPT: 84210
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Test Details

Synonyms

  • Pyruvate

Use

Increased pyruvic acid levels have been associated with diabetes mellitus, vitamin deficiencies, uremia, congestive heart failure, liver diseases, muscular dystrophy, thiamine deficiency, and neoplastic conditions. Pyruvic acid is useful in assessing oxygen deprivation and provides an index of the severity of circulatory failure.

Limitations

There appears to be no adequate means of preserving the pyruvic acid level (including the use of sodium fluoride), except immediate precipitation of blood proteins. Because of the time required to obtain serum or plasma from drawn blood, the pyruvic acid values observed with plasma are likely to be different from the true initial values; therefore, the use of whole blood with subsequent protein precipitation for pyruvic acid determination is recommended.

Methodology

Pyruvate − lactate; spectrophotometry

Reference Interval

0.3−0.7 mg/dL

Specimen Requirements

Specimen

Perchloric acid supernatant from oxalated whole blood

Volume

0.5 mL (clear supernatant)

Minimum Volume

0.2 mL (clear supernatant)

Container

Gray-top (sodium fluoride) tube

Patient Preparation

Patient should be in a fasting and resting state (should not exercise).

Collection

Draw blood into chilled gray-top tube. Immediately after blood is drawn, add 2 mL of whole blood from the gray-top tube to 2 mL of 8% perchloric acid (7 mL of 70% perchloric acid diluted to 100 mL with deionized water). Note: Use 1:1 blood (ie, equal parts) to 8% perchloric ratio if less blood is obtained. Shake mixture vigorously for about 30 seconds. Refrigerate for five minutes to ensure complete protein precipitation. Centrifuge 5 to 10 minutes at approximately 1500xg. Immediately transfer the clear supernatant to a transport tube. Label the transport tube as "supernatant" and send to the laboratory. Note: A second centrifugation of the supernatant may be necessary to obtain a clear protein-free solution.

Storage Instructions

Maintain the perchloric acid supernatant refrigerated.

Stability Requirements

Temperature

Period

Room temperature

5 days

Refrigerated

7 days

Frozen

7 days

Freeze/thaw cycles

Stable x3

Causes for Rejection

Whole blood received; gray-top tube not prepared with perchloric acid

Clinical Information

Aftercare

Solutions of perchloric acid can become explosive if allowed to dry. Always rinse glassware and working surfaces copiously with water.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
004788 Pyruvic Acid, Blood 2905-8 004788 Pyruvic Acid, Blood mg/dL 2905-8
Reflex Table for Pyruvic Acid, Blood
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 004789 Disclaimer: 004789 Disclaimer: N/A

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