Protein Electrophoresis, Serum and Quantitative Free κ and λ Light Chains (FLC) Plus Ratio, Serum

CPT: 83883(x2); 84155; 84165
Updated on 1/24/2018
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Test Details

Synonyms

  • Electrophoresis, Serum
  • FLC, Serum
  • Free Light Chains, Quantitative, Serum
  • Kappa Free Light Chains, Quantitative, Serum
  • Kappa/Lambda Free Light Chains, Quantitative, Serum
  • Kappa:Lambda Free Light Chains Ratio, Quantitative, Serum
  • Lambda Free Light Chains, Quantitative, Serum
  • Light Chains, Free κ and Free λ, Quantitative, Serum

Test Includes

Serum protein electrophoresis with relative percentages and quantitation of albumin; α1-, α2-, β-, and γ-globulin fractions; graph of fractions; quantitation of total protein and M-spike if present; quantitation of serum free κ and λ light chains plus κ:λ ratio

Use

Detect and identify monoclonal immunoglobulin gammopathies

Methodology

Protein electrophoresis: agarose media; free κ and λ light chains: immunologic quantitation

Additional Information

Elevated serum levels of monoclonal FLC are associated with malignant plasma cell proliferation (eg, multiple myeloma), primary amyloidosis, and light chain deposition disease. Raised serum levels of polyclonal FLC may be associated with autoimmune diseases such as systemic lupus erythematosus.

Specimen Requirements

Specimen

Serum

Volume

2.5 mL

Minimum Volume

1.2 mL

Container

Red-top tube or gel-barrier tube

Collection

Separate serum from cells.

Storage Instructions

PE: Stable when covered at room temperature 25°C for four days; stable refrigerated at 2°C to 8°C for two weeks; stable frozen at -20°C for six months.FLC: Refrigerate; samples are stable for 21 days at 2°C to 8°C. For longer storage, freeze at -20°C.

Causes for Rejection

Lipemia; hemolysis; plasma specimen

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
121210 PE and FLC, Serum 001073 Protein, Total, Serum g/dL 2885-2
121210 PE and FLC, Serum 001488 Albumin g/dL 2862-1
121210 PE and FLC, Serum 001489 Alpha-1-Globulin g/dL 2865-4
121210 PE and FLC, Serum 001490 Alpha-2-Globulin g/dL 2868-8
121210 PE and FLC, Serum 001491 Beta Globulin g/dL 2871-2
121210 PE and FLC, Serum 001492 Gamma Globulin g/dL 2874-6
121210 PE and FLC, Serum 001493 M-Spike g/dL 33358-3
121210 PE and FLC, Serum 011052 Globulin, Total g/dL 10834-0
121210 PE and FLC, Serum 011149 A/G Ratio 1759-0
121210 PE and FLC, Serum 011150 Please note: N/A
121210 PE and FLC, Serum 001498 PDF 11502-2
121210 PE and FLC, Serum 121138 Free Kappa Lt Chains,S mg/L 36916-5
121210 PE and FLC, Serum 121139 Free Lambda Lt Chains,S mg/L 33944-0
121210 PE and FLC, Serum 121140 Kappa/Lambda Ratio,S 48378-4

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The LOINC® codes are copyright © 1994-2018, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf