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- Loa loa Smear
- Plasmodium Smear
- Wuchereria Smear
- Blood Smear for Parasites
- Microfilarial Smear
- Parasitology Examination for Malaria
Establish the diagnosis of Plasmodium or other parasitic infection; diagnose malarial parasitic infestation of blood; evaluate febrile disease of unknown origin
One negative result does not rule out the possibility of parasitic infestation. If protozoal, filarial, or trypanosomal infection is strongly suspected, test should be performed at least three times with samples obtained at different times in the fever cycle.
Wright stain; microscopic examination of thick and thin peripheral blood smears stained with Romanovsky dye (in particular Giemsa). Thick films are more difficult to interpret but greatly increase sensitivity (by concentrating cells and organisms). Thick smears require considerable experience with malaria, as they increase the number of cells examined in a given time period by a factor of about 12.1
No organisms identified
Proper therapy depends on identification of the specific variety of malaria parasite. Release of trophozoites and RBC debris results in a febrile response. Periodicity of fever correlates with type of malaria (see table). Organisms are most likely to be detected just before onset of fever, which is predictable in many cases. Sampling immediately upon onset of fever is the most desirable time to obtain blood. Alternatively, in cases negative by these means but with a strong clinical history, multiple sampling at different times in the fever cycle may prove successful.
Infected RBC Enlarged
Presence of Schüffner Dots
Presence of Maurer Dots
Multiple Parasites per RBC
Parasite With Double Chromatin Dots
Parasite With Sausage- Shaped Gametocytes
Smears made from fresh whole capillary (fingerstick) blood and/or capillary blood in EDTA (Microtainer™), or 3 to 5 mL fresh whole venous blood in EDTA
Films (two thin and two thick) or 3 to 5 mL fresh whole venous blood in EDTA
Glass slide, lavender-top (EDTA) tube
Prepare sterile venipuncture site
Maintain EDTA whole blood specimen at room temperature for no longer than 24 to 48 hours.
Causes for Rejection
Specimen clotted; improper labeling
|Order Code||Order Code Name||Order Loinc||Result Code||Result Code Name||UofM||Result LOINC|
|008185||Parasite Exam, Blood||24429-3||008185||Parasite Exam, Blood||24429-3|