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17-OH Progesterone, LC/MS, ACTH Stimulation
- 17-OHP ACTH Stimulation
This test may involve significant risk and should be performed only by qualified personnel who are familiar with the test and who have taken adequate precautions to protect the safety of the patient. Every effort has been made to ensure accuracy in these recommendations, but clinicians must use their judgment and refer to specific pharmaceutical resources to determine appropriate drug dosages for their patients.
Liquid chromatography/tandem mass spectrometry (LC/MS-MS)
Serum or plasma
1 mL (each specimen)
0.5 mL (Note: This volume does not allow for repeat testing.)
Red-top tube, gel-barrier tube, or lavender-top (EDTA) tube
If tube other than gel-barrier tube is used, transfer separated serum or plasma to a plastic transport tube. Note: Label each tube with patient's name, and date and time of collection. Submit specimens simultaneously on the same test request form.
Draw blood for baseline 17-OH progesterone. Inject cosyntropin 250 μg IM or IV (if IV, dilute cosyntropin in 2 to 5 mL of sterile saline and inject over two minutes). Draw blood for stimulated 17-OH progesterone 60 minutes after. See the online Endocrine Appendix: ACTH Stimulation for expected values for various steroids at baseline and after ACTH stimulation.
|Order Code||Order Code Name||Order Loinc||Result Code||Result Code Name||UofM||Result LOINC|
|070624||17-OH Progesterone ACTH Stimu.||070625||17-OH Progesterone Baseline||ng/dL||27996-8|
|070624||17-OH Progesterone ACTH Stimu.||070626||17-OH Progesterone Stimulated||ng/dL||14114-3|