17-OH Progesterone, LC/MS, ACTH Stimulation

CPT: 83498(x2)
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Test Details


  • 17-OHP ACTH Stimulation


This test may involve significant risk and should be performed only by qualified personnel who are familiar with the test and who have taken adequate precautions to protect the safety of the patient. Every effort has been made to ensure accuracy in these recommendations, but clinicians must use their judgment and refer to specific pharmaceutical resources to determine appropriate drug dosages for their patients.


Liquid chromatography/tandem mass spectrometry (LC/MS-MS)

Specimen Requirements


Serum or plasma


1 mL (each specimen)

Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)


Red-top tube, gel-barrier tube, or lavender-top (EDTA) tube


If tube other than gel-barrier tube is used, transfer separated serum or plasma to a plastic transport tube. Note: Label each tube with patient's name, and date and time of collection. Submit specimens simultaneously on the same test request form.

Storage Instructions


Stability Requirements



Room temperature

7 days


7 days


14 days

Freeze/thaw cycles

Stable x3

Clinical Information

Special Instructions

Draw blood for baseline 17-OH progesterone. Inject cosyntropin 250 μg IM or IV (if IV, dilute cosyntropin in 2 to 5 mL of sterile saline and inject over two minutes). Draw blood for stimulated 17-OH progesterone 60 minutes after. See the online Endocrine Appendix: ACTH Stimulation for expected values for various steroids at baseline and after ACTH stimulation.


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
070624 17-OH Progesterone ACTH Stimu. 070625 17-OH Progesterone Baseline ng/dL 27996-8
070624 17-OH Progesterone ACTH Stimu. 070626 17-OH Progesterone Stimulated ng/dL 14114-3

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