17-OH Progesterone, LC/MS, ACTH Stimulation

CPT: 83498(x2)
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Test Details

Synonyms

  • 17-OHP ACTH Stimulation

Use

This test may involve significant risk and should be performed only by qualified personnel who are familiar with the test and who have taken adequate precautions to protect the safety of the patient. Every effort has been made to ensure accuracy in these recommendations, but clinicians must use their judgment and refer to specific pharmaceutical resources to determine appropriate drug dosages for their patients.

Methodology

Liquid chromatography/tandem mass spectrometry (LC/MS-MS)

Specimen Requirements

Specimen

Serum or plasma

Volume

1 mL (each specimen)

Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)

Container

Red-top tube, gel-barrier tube, or lavender-top (EDTA) tube

Collection

If tube other than gel-barrier tube is used, transfer separated serum or plasma to a plastic transport tube. Note: Label each tube with patient's name, and date and time of collection. Submit specimens simultaneously on the same test request form.

Storage Instructions

Refrigerate.

Stability Requirements

Temperature

Period

Room temperature

7 days

Refrigerated

7 days

Frozen

14 days

Freeze/thaw cycles

Stable x3

Clinical Information

Special Instructions

Draw blood for baseline 17-OH progesterone. Inject cosyntropin 250 μg IM or IV (if IV, dilute cosyntropin in 2 to 5 mL of sterile saline and inject over two minutes). Draw blood for stimulated 17-OH progesterone 60 minutes after. See the online Endocrine Appendix: ACTH Stimulation for expected values for various steroids at baseline and after ACTH stimulation.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
070624 17-OH Progesterone ACTH Stimu. 070625 17-OH Progesterone Baseline ng/dL 27996-8
070624 17-OH Progesterone ACTH Stimu. 070626 17-OH Progesterone Stimulated ng/dL 14114-3
070624 17-OH Progesterone ACTH Stimu. 070627 17-OH Progesterone (2 Spec) N/A

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The LOINC® codes are copyright © 1994-2017, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf