5′ Nucleotidase

CPT: 83915
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Expected Turnaround Time

3 - 4 days


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Specimen Requirements


Specimen

Serum (EDTA or heparin plasma may be used.)


Volume

1 mL


Minimum Volume

0.2 mL (Note: This volume does not allow for repeat testing.)


Container

Red-top tube or gel-barrier tube for serum; lavender-top (EDTA) tube or green-top (heparin) tube for plasma


Storage Instructions

Room temperature


Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3


Causes for Rejection

Hemolysis


Test Details


Use

5′ nucleotidase is used to investigate the origin of increased serum alkaline phosphatase. It is a liver-related enzyme used to work up cholestatic/biliary obstruction. It parallels the increases of alkaline phosphatase and leucine aminopeptidase in hepatobiliary diseases, but is not usually elevated in skeletal disorders such as Paget disease of bone. It is increased with metastatic neoplasia in liver and in primary biliary cirrhosis, biliary obstruction from calculi and tumor.


Methodology

Kinetic − 340 nm


Reference Interval

See table.

Age

Male (IU/L)

Female (IU/L)

0 to 1 y

0−6

0−18

2 to 12 y

0−7

0–7

13 to 17 y

0−5

0−5

18 to 30 y

0–5

0–7

31 to 50 y

0−10

0−10

51 to 70 y

0−11

0−11

71 to 80 y

0−13

0−13

>80 y

0−18

0−15


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
001701 5' Nucleotidase 1690-7 001701 5' Nucleotidase IU/L 1690-7

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