Norovirus Detection, Real-time PCR

CPT: 87798(x2)
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Synonyms

  • Human Calicivirus
  • Norwalk Virus

Expected Turnaround Time

4 - 5 days


Related Documents


Specimen Requirements


Specimen

Stool


Volume

1 mL


Minimum Volume

0.2 mL


Container

Sterile container or Para-Pak® Orange


Storage Instructions

Refrigerate. Samples in Cary-Blair medium (Para-Pak® Orange) can be kept at room temperature.


Stability Requirements

Temperature

Period

Room temperature

Sterile container: 7 days;

Cary-Blair medium: 5 days

Refrigerated

Sterile container: 7 days;

Cary-Blair medium: 14 days

Frozen

Sterile container: 7 days;

Cary-Blair medium: Unstable


Causes for Rejection

Quantity not sufficient for analysis; specimen too old; leaking or broken tube; frozen sample in Cary-Blair medium (Para-Pak® Orange)


Test Details


Use

This test is intended to be used as an aid in the diagnosis of infections caused by noroviruses, and to differentiate viruses belonging to Norovirus genogroup 1 (eg, Norwalk virus) and from those belonging to Norovirus genogroup 2 (eg, Snow Mountain agent).


Methodology

Real-time reverse transcriptase polymerase chain reaction (RT-PCR)


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
138307 Norovirus, RT-PCR 88701-8 139356 Norovirus GI 54905-5
138307 Norovirus, RT-PCR 88701-8 139357 Norovirus GII 54906-3

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