Natural Killer Cell Surface Antigen (CD3-CD56+ Marker Analysis)

CPT: 86357
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Test Details

Test Includes

Percentage CD3-CD56+ natural killers (NK+); absolute CD3-CD56+ natural killers (NK+); absolute lymphocyte count

Use

Determine levels of natural killer cells in circulation; monitor immune stimulation therapy

Methodology

Flow cytometry

Reference Interval

Reference intervals for adults have been established by the laboratory. See table.

CD3_CD56+ Reference Intervals for Lymphocyte Immunophenotyping

Age

95% Confidence Interval

Percentage

Cells/mm3

Minimum

Maximum

Minimum

Maximum

Pediatric reference intervals are from Comans-Bitter WM, de Groot R, van den Beemd R, et al. Immunophenotyping of blood lymphocytes in childhood. Reference values for lymphocyte subpopulations. J Pediatrics. 1997 Mar; 130(3):388-393.

Neonates

6

58

100

1900

1 wk to 2 m

3

23

200

1400

2 to 5 m

2

14

100

1300

5 to 9 m

2

13

100

1000

9 to 15 m

3

17

200

1200

15 to 24 m

3

16

100

1400

2 to 5 y

4

23

100

1000

5 to 10 y

4

26

90

900

10 to 16 y

6

27

70

1200

Adults

1

19

24

406

Additional Information

Natural killer (NK) cells are large granular lymphocytes that mediate MHC-unrestricted cytotoxicity against virus-infected and malignant cells and manufacture a number of cytokines following stimulation of the immune system.

Specimen Requirements

Specimen

Whole blood

Volume

Fill tube(s) to capacity.

Container

Lavender-top (EDTA) tube and yellow-top (ACD-A) or (ACD-B) tube

Collection

Invert tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimen so it will arrive in the laboratory within 48 hours of collection. Indicate date and time of venipuncture on the tube(s) and on the test request form.

Storage Instructions

Maintain specimen at room temperature.

Stability Requirements

Temperature

Period

Room temperature

2 days

Refrigerated

Unstable

Frozen

Unstable

Freeze/thaw cycles

Unstable

Causes for Rejection

Hemolysis; specimen refrigerated or frozen; clotted specimen; contaminated specimen

Clinical Information

References

Stevens RA, Lempicki RA, Natarajan V, et al. General immunologic evaluation of patients with immunodeficiency virus infection. In: Detrick B, Hamilton RG, Folds JD, et al, eds. Manual of Molecular and Clinical Laboratory Immunology. 7th ed. Washington, DC: ASM Press;2006:chap 95.

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