Natural Killer Cell Surface Antigen (CD3-CD56+ Marker Analysis)

CPT: 86357
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Test Details

Test Includes

Percentage CD3-CD56+ natural killers (NK+); absolute CD3-CD56+ natural killers (NK+); absolute lymphocyte count

Use

Determine levels of natural killer cells in circulation; monitor immune stimulation therapy

Methodology

Flow cytometry

Reference Interval

Reference intervals for adults have been established by the laboratory. See table.

CD3_CD56+ Reference Intervals for Lymphocyte Immunophenotyping

Age

95% Confidence Interval

Percentage

Cells/mm3

Minimum

Maximum

Minimum

Maximum

Pediatric reference intervals are from Comans-Bitter WM, de Groot R, van den Beemd R, et al. Immunophenotyping of blood lymphocytes in childhood. Reference values for lymphocyte subpopulations. J Pediatrics. 1997 Mar; 130(3):388-393.

Neonates

6

58

100

1900

1 wk to 2 m

3

23

200

1400

2 to 5 m

2

14

100

1300

5 to 9 m

2

13

100

1000

9 to 15 m

3

17

200

1200

15 to 24 m

3

16

100

1400

2 to 5 y

4

23

100

1000

5 to 10 y

4

26

90

900

10 to 16 y

6

27

70

1200

Adults

1

19

24

406

Additional Information

Natural killer (NK) cells are large granular lymphocytes that mediate MHC-unrestricted cytotoxicity against virus-infected and malignant cells and manufacture a number of cytokines following stimulation of the immune system.

Specimen Requirements

Specimen

Whole blood

Volume

Fill tube(s) to capacity.

Container

Lavender-top (EDTA) tube and yellow-top (ACD-A) or (ACD-B) tube

Collection

Invert tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimen so it will arrive in the laboratory within 48 hours of collection. Indicate date and time of venipuncture on the tube(s) and on the test request form.

Storage Instructions

Maintain specimen at room temperature.

Stability Requirements

Temperature

Period

Room temperature

2 days

Refrigerated

Unstable

Frozen

Unstable

Freeze/thaw cycles

Unstable

Causes for Rejection

Hemolysis; specimen refrigerated or frozen; clotted specimen; contaminated specimen

Clinical Information

References

Stevens RA, Lempicki RA, Natarajan V, et al. General immunologic evaluation of patients with immunodeficiency virus infection. In: Detrick B, Hamilton RG, Folds JD, et al, eds. Manual of Molecular and Clinical Laboratory Immunology. 7th ed. Washington, DC: ASM Press;2006:chap 95.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
505016 Natural Killer Cell Surface Ag 506730 Ab NK (CD56/16) /uL 42188-3
505016 Natural Killer Cell Surface Ag 506729 % NK (CD56/16) % 42189-1
505016 Natural Killer Cell Surface Ag 005025 WBC x10E3/uL 6690-2
505016 Natural Killer Cell Surface Ag 005033 RBC x10E6/uL 789-8
505016 Natural Killer Cell Surface Ag 005041 Hemoglobin g/dL 718-7
505016 Natural Killer Cell Surface Ag 005058 Hematocrit % 4544-3
505016 Natural Killer Cell Surface Ag 015065 MCV fL 787-2
505016 Natural Killer Cell Surface Ag 015073 MCH pg 785-6
505016 Natural Killer Cell Surface Ag 015081 MCHC g/dL 786-4
505016 Natural Killer Cell Surface Ag 105007 RDW % 788-0
505016 Natural Killer Cell Surface Ag 015172 Platelets x10E3/uL 777-3
505016 Natural Killer Cell Surface Ag 015107 Neutrophils % 770-8
505016 Natural Killer Cell Surface Ag 015123 Lymphs % 736-9
505016 Natural Killer Cell Surface Ag 015131 Monocytes % 5905-5
505016 Natural Killer Cell Surface Ag 015149 Eos % 713-8
505016 Natural Killer Cell Surface Ag 015156 Basos % 706-2
505016 Natural Killer Cell Surface Ag 115398 Immature Cells N/A
505016 Natural Killer Cell Surface Ag 015909 Neutrophils (Absolute) x10E3/uL 751-8
505016 Natural Killer Cell Surface Ag 015917 Lymphs (Absolute) x10E3/uL 731-0
505016 Natural Killer Cell Surface Ag 015925 Monocytes(Absolute) x10E3/uL 742-7
505016 Natural Killer Cell Surface Ag 015933 Eos (Absolute) x10E3/uL 711-2
505016 Natural Killer Cell Surface Ag 015941 Baso (Absolute) x10E3/uL 704-7
505016 Natural Killer Cell Surface Ag 015108 Immature Granulocytes % 38518-7
505016 Natural Killer Cell Surface Ag 015911 Immature Grans (Abs) x10E3/uL 51584-1
505016 Natural Killer Cell Surface Ag 015945 NRBC % 58413-6
505016 Natural Killer Cell Surface Ag 015180 Hematology Comments: 18314-5
505016 Natural Killer Cell Surface Ag 000000 HIV Confidential N/A
Reflex Table for Immature Granulocytes
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 015111 Please note: 015111 Please note: N/A
Reflex Table for Immature Grans (Abs)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 015111 Please note: 015111 Please note: N/A
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115247 Neutrophils % 23761-0
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115250 Lymphs % 737-7
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115251 Monocytes % 744-3
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115252 Eos % 714-6
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115253 Basos % 707-0
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115398 Immature Cells N/A
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115254 Neutrophils Absolute X10E3/uL 753-4
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115255 Lymphs (Absolute) X10E3/uL 732-8
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115256 Monocytes(Absolute) X10E3/uL 743-5
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115257 Eos (Absolute Value) X10E3/uL 712-0
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115258 Baso(Absolute) X10E3/uL 705-4
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115260 Differential Comment 18723-7
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 005034 RBC Comment 6742-1
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 015171 Platelet Comment 9317-9

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