Natural Killer Cell and Activated T-Cell Profile/IL-2r

CPT: 86356; 86357; 86359
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Test Details

Synonyms

  • CD25
  • Interleukin-2 Receptor
  • TAC Antigen

Test Includes

Percentage CD3+; absolute CD3+; percentage CD3+CD25+ (IL-2 receptor); absolute CD3+CD25+; percentage CD3-CD56+ (NK); total CD3-CD56+ (NK); CBC with differential; total lymphocyte count

Use

Assess changes in T-lymphocyte surface markers that may indicate immune stimulation

Limitations

This test was developed, and its performance characteristics determined, by LabCorp. It has not been cleared or approved by the US Food and Drug Administration (FDA).

Methodology

Flow cytometry

Reference Interval

Reference intervals for adults have been established by the laboratory. See table.

Adult Reference Intervals for Lymphocyte Immunophenotyping

CD

95% Confidence Interval

Percentage

Cells/mm3

Minimum

Maximum

Minimum

Maximum

CD3+

58

86

622

2402

CD3+CD25+

5

26

79

535

CD3-CD56+

1

19

24

406

Additional Information

Natural killer (NK) cells are large granular lymphocytes that mediate MHC-unrestricted cytotoxicity against virus-infected and malignant cells and manufacture a number of cytokines following stimulation of the immune system.

The combination of IL-2 and T-cell markers can be used to assess the state of activation of T cells. NK cells are responsible for the destruction of virally infected cells, tumor cells, and antibody-dependent cellular cytotoxicity.

Specimen Requirements

Specimen

Whole blood

Volume

Fill tube(s) to capacity.

Container

Lavender-top (EDTA) tube and yellow-top (ACD-A) or (ACD-B) tube

Collection

Invert tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimen so it will arrive in the laboratory within 48 hours of collection. Indicate date and time of venipuncture on the tube(s) and on the test request form.

Storage Instructions

Maintain specimen at room temperature.

Stability Requirements

Temperature

Period

Room temperature

2 days

Refrigerated

Unstable

Frozen

Unstable

Freeze/thaw cycles

Unstable

Causes for Rejection

Hemolysis; specimen refrigerated or frozen; clotted specimen; contaminated specimen

Clinical Information

References

Caligiuri M, Murray C, Buchwald D, et al. Phenotypic and functional deficiency of natural killer cells in patients with chronic fatigue syndrome. J Immunol. 1987 Nov 15; 139(10):3306-3313. 2824604
Landay AL, Jessop C, Lennette ET, Levy JA. Chronic fatigue syndrome: Clinical condition associated with immune activation. Lancet. 1991 Sep 21; 338(8769):707-712.1679864
Lanier L, Jackson A. Monoclonal antibodies: Differentiation antigens expressed on leukocytes. In: Rose N, deMacario E, Fahey J, et al, eds. Manual of Clinical Laboratory Immunology. 4th ed. Washington, DC: ASM Press;1992:chap 23.
Rich RR, ed. Clinical Immunology: Principles and Practice. St Louis, Mo: Mosby-Year Book;1996:chap 87.

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The LOINC® codes are copyright © 1994-2018, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf