Leptin, Serum or Plasma

CPT: 83520
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Test Details

Use

Assessment of obesity, delayed puberty, and infertility

Limitations

Results of this test are labeled for research purposes only by the assay's manufacturer. The performance characteristics of this assay have not been established by the manufacturer. The result should not be used for treatment or for diagnostic purposes without confirmation of the diagnosis by another medically established diagnostic product or procedure. The performance characteristics were determined by LabCorp.

Methodology

Enzyme immunoassay (EIA)

Reference Interval

See tables.

Female Ranges <20 by Body Mass Index (BMI)

BMI

Tanner Stage 1−2

Tanner Stage 3−4

Tanner Stage 5

Blum WF, Juul A. Reference ranges of leptin levels according to body mass index, gender and development stage. In: Blum WF, Kiess WF, Rascher W, eds. Leptin−The Voice of Adipose Tissue. Heidelberg, Germany: Johann Ambrosius Barth Verlag;1997:319-326.

11

0.3−1.5

0.4−1.3

0.7−2.7

12

0.4−1.9

0.5−1.6

0.8−3.2

13

0.5−2.4

0.7−2.1

0.9−3.7

14

0.6−3.1

0.8−2.6

1.0−4.3

15

0.8−3.9

1.1−3.3

1.2−5.0

16

1.1−5.0

1.3−4.2

1.4−5.8

17

1.4−6.5

1.7−5.3

1.6−6.7

18

1.7−8.3

2.1−6.7

1.9−7.8

19

2.2−10.7

2.7−8.5

2.2−9.1

20

2.9−13.7

3.4−10.7

2.6−10.5

21

3.7−17.6

4.3−13.6

3.0−12.3

22

4.7−22.6

5.5−17.2

3.5−14.2

23

6.0−29.0

6.9−21.8

4.0−16.6

24

7.8−37.2

8.8−27.6

4.7−19.3

25

10.0−47.8

11.1−34.9

5.5−22.4

26

12.8−61.4

14.0−44.2

6.4−26.0

27

16.4−78.8

17.7−56.0

7.4−30.3

28

21.5−101.0

22.5−70.9

8.6−35.2

29

27.0−130.0

28.4−89.7

10.0−40.9

30

36.0−114.0

11.6−47.6

31

45.6−144.0

13.5−55.3

32

57.7−144.0

15.7−64.4

33

18.3−74.9

34

21.2−87.0

35

24.7−101.0

36

28.7−118.0

37

33.4−137.0

Male Ranges <20 by Body Mass Index (BMI)

BMI

Tanner Stage 1−2

Tanner Stage 3−4

Tanner Stage 5

Blum WF, Juul A. Reference ranges of leptin levels according to body mass index, gender and development stage. In: Blum WF, Kiess WF, Rascher W, eds. Leptin−The Voice of Adipose Tissue. Heidelberg, Germany: Johann Ambrosius Barth Verlag;1997:319-326.

11

0.1−0.7

0.1−0.6

0.1−0.5

12

0.2−0.9

0.1−0.7

0.1−0.5

13

0.2−1.2

0.1−0.9

0.1−0.6

14

0.3−1.6

0.1−1.1

0.1−0.7

15

0.4−2.0

0.1−1.3

0.1−0.8

16

0.5−2.7

0.2−1.6

0.1−1.0

17

0.6−3.5

0.2−2.0

0.1−1.1

18

0.8−4.6

0.2−2.5

0.2−1.3

19

1.0−6.0

0.3−3.0

0.2−1.5

20

1.4−7.9

0.3−3.7

0.2−1.7

21

1.8−10.4

0.4−4.6

0.2−2.0

22

2.3−13.6

0.5−5.6

0.3−2.3

23

3.1−17.8

0.6−6.9

0.3−2.7

24

4.0−23.3

0.8−8.5

0.4−3.1

25

5.2−30.6

1.0−10.5

0.4−3.6

26

6.9−40.1

1.2−12.9

0.5−4.2

27

9.0−52.5

1.5−15.8

0.6−4.8

28

11.8−68.9

1.8−19.4

0.6−5.6

29

15.5−90.3

2.2−23.9

0.7−6.5

30

20.3−118.0

2.7−29.4

0.9−7.5

31

3.3−36.2

1.0−8.7

32

4.1−44.5

1.2−10.0

33

5.0−54.7

1.3−11.6

34

6.2−67.2

1.5−13.4

35

7.6−82.6

1.8−15.6

36

9.4−101.0

2.1−18.0

37

11.5−124.0

2.4−20.8

38

2.8−24.1

39

3.2−27.9

40

3.7−32.3

Female Ranges >19 by Body Mass Index (BMI)

BMI

Range

BMI

Range

Blum WF, Juul A. Reference ranges of leptin levels according to body mass index, gender and development stage. In: Blum WF, Kiess WF, Rascher W, eds. Leptin−The Voice of Adipose Tissue. Heidelberg, Germany: Johann Ambrosius Barth Verlag;1997:319-326.

11

0.7−3.6

24

4.4−24.2

12

0.8−4.2

25

5.1−28.0

13

0.9−4.8

26

5.9−32.4

14

1.0−5.6

27

6.8−37.5

15

1.2−6.5

28

7.9−43.5

16

1.4−7.5

29

9.1−50.4

17

1.6−8.7

30

10.6−58.3

18

1.8−10.0

31

12.2−67.5

19

2.1−11.6

32

14.1−78.2

20

2.4−13.4

33

16.4−90.5

21

2.8−15.6

34

19.0−105.0

22

3.3−18.0

35

22.0−121.0

23

3.8−20.9

36

25.4−141.0

Male Ranges >19 by Body Mass Index (BMI)

BMI

Range

BMI

Range

Blum WF, Juul A. Reference ranges of leptin levels according to body mass index, gender and development stage. In: Blum WF, Kiess WF, Rascher W, eds. Leptin−The Voice of Adipose Tissue. Heidelberg, Germany: Johann Ambrosius Barth Verlag;1997:319-326.

11

0.1−0.4

25

1.1−9.6

12

0.1−0.6

26

1.3−12.0

13

0.1−0.7

27

1.6−14.9

14

0.1−0.9

28

2.0−18.6

15

0.1−1.1

29

2.5−23.2

16

0.2−1.3

30

3.2−28.9

17

0.2−1.7

31

3.9−36.0

18

0.2−2.1

32

4.9−44.9

19

0.3−2.6

33

6.1−55.8

20

0.4−3.2

34

7.6−69.6

21

0.4−4.0

35

9.5−86.7

22

0.5−5.0

36

11.8−108.0

23

0.8−6.2

37

14.8−135.0

24

0.9−7.7

Additional Information

Leptin is a 16-kilodalton protein that was first identified when it was cloned in 1994.1,2 Humans and mice with defects in the gene that codes for leptin, referred to as the obese or OB gene, tend to become morbidly obese.2 Obese mice with the ob/ob defect lose weight when treated with exogenous leptin.2 Leptin is produced primarily by white adipose tissue and blood levels correlated with body fat stores.2,3 Increased leptin levels stimulate the central nervous system to reduce appetite and increase energy expenditure.2,3 Leptin is thought to play an important role in the body's response to food deprivation or starvation.3,4

Ongoing research indicates that leptin is also involved in a diverse array of physiologic functions beyond control of food intake and energy expenditure.2,3 A role for leptin has been implicated in the control of angiogenesis, hematopoiesis, immunity and bone formation, and a number of other functions.2 Leptin is thought to play a role in normal sexual development and in reproduction.4,5 Humans and mice with genetic absence of leptin fail to complete puberty and increased leptin levels in mice lead to early puberty.4 Studies also suggest that leptin levels affect fertility in females and may be involved in the development of normal pregnancy.4,5 During pregnancy, the placenta produces leptin, and maternal circulating levels during the second and third trimesters are approximately twice the level of the nonpregnant state.4,5

Specimen Requirements

Specimen

Serum or plasma

Volume

0.2 mL

Minimum Volume

0.1 mL (Note: This volume does not allow for repeat testing.)

Container

Red-top tube, gel-barrier tube, or lavender-top (EDTA) tube

Collection

Transfer serum or plasma from cells and transfer to a plastic transport tube.

Storage Instructions

Room temperature

Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

30 days

Frozen

21 days

Freeze/thaw cycles

Stable x3

Causes for Rejection

Gross hemolysis; gross lipemia

Clinical Information

Footnotes

1. Zhang Y, Proenca R, Maffei M, Barone M, Leopold L, Friedman JM. Positional cloning of the mouse obese gene and its human homologue. Nature. 1994 Dec 1; 372(6505):425-432. 7984236
2. Trayhurn P, Hoggard N, Mercer JG, Rayner DV. Leptin: Fundamental aspects. Int J Obes Relat Metab Disord. 1999 Feb; 23(Suppl 1):22-28 (review). 10193858
3. Van Gaal LF, Wauters MA, Mertens IL, Considine RV, De Leeuw IH. Clinical endocrinology of human leptin. Int J Obes Relat Metab Disord. 1999 Feb; 23(Suppl 1):29-36. 10193859
4. Reitman ML, Bi S, Marcus-Samuels B, Gavrilova O. Leptin and its role in pregnancy and fetal development−An overview. Biochem Soc Trans. 2001 May; 29(Pt 2):68-72 (review). 11356129
5. Hoggard N, Haggarty P, Thomas L, Lea RG. Leptin expression in placental and fetal tissues: Does leptin have a functional role? Biochem Soc Trans. 2001 May; 29(Pt 2):57-63 (review).11356127

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
146712 Leptin, Serum 21365-2 146713 Leptin, Serum ng/mL 21365-2

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The LOINC® codes are copyright © 1994-2018, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf