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Expected Turnaround Time
Within 1 day
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Serum (preferred) or plasma
Red-top tube, gel-barrier tube, or green-top (heparin) tube; do not use EDTA plasma.
Separate serum or plasma from cells within 45 minutes of collection.
Maintain specimen at room temperature.1
Causes for Rejection
Plasma specimens collected in EDTA, oxalate, or citrated tubes; gross hemolysis; gross bacterial contamination; improper labeling
Elevated serum levels of LDH have been observed in a variety of disease states. The highest levels are seen in patients with megaloblastic anemia, disseminated carcinoma, and shock. Moderate increases occur in muscular disorders, renal diseases, and cirrhosis. Mild increases in LDH activity have been reported in cases of myocardial or pulmonary infarction, leukemia, hemolytic anemia, and nonviral hepatitis.2
Hemolysis elevates LDH results, oxalate inhibits LDH, and ascorbic acid can decrease LDH values.
Enzymatic, colorimetric, UV
0 to 7 d
8 to 30 d
1 to 11 m
1 to 3 y
4 to 6 y
7 to 9 y
10 to 12 y
13 to 15 y
16 to 17 y
In infectious mononucleosis, LD is usually more elevated than AST, and there is usually an isomorphic pattern of LD isoenzymes. In viral hepatitis, by contrast, AST and ALT (the transaminases) are much more increased than is LD, about three or more times higher than total LD, and LD5 is high. The differential diagnosis of acute infarct of myocardium includes pericarditis and angina, entities in which enzymes are usually not substantially increased. Bovine or porcine heparin therapy can cause increases of AST, ALT, and LD, with elevated LD hepatic fractions.2
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