Immunofixation (IFE), Serum

CPT: 82784(x3) (IgG, IgA, IgM); 86334 (immunofixation electrophoresis)
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Test Details

Synonyms

  • Electrophoresis, Serum, IFE
  • Immunoelectrophoresis, Serum

Test Includes

Quantitation of immunoglobulins IgA, IgG, and IgM; IFE interpretation of heavy- and light-chain patterns in the serum

Use

Detect and identify monoclonal immunoglobulin gammopathies

Methodology

Electrophoresis followed by immunodiffusion against monospecific antisera to immunoglobulin and individual heavy and light chains (IFE); turbidimetric quantitation of IgA, IgG, and IgM

Reference Interval

Normal pattern

Additional Information

Immunofixation electrophoresis of serum or urine is most often ordered to evaluate a monoclonal globulin detected in a protein electrophoresis or to delineate a possible lymphoproliferative process, particularly myeloma. This procedure will characterize the specific light and heavy chain components of a monoclonal protein.

Specimen Requirements

Specimen

Serum

Volume

2 mL

Minimum Volume

1 mL

Container

Red-top tube or gel-barrier tube

Patient Preparation

Patient should be fasting.

Collection

Separate serum from red cells.

Storage Instructions

Room temperature

Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3

Causes for Rejection

Gross hemolysis; gross lipemia

Clinical Information

Special Instructions

State patient's age and clinical diagnosis on the test request form.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
001685 Immunofixation, Serum 001795 Immunofixation Result, Serum 14895-7
001685 Immunofixation, Serum 001776 Immunoglobulin G, Qn, Serum mg/dL 2465-3
001685 Immunofixation, Serum 001784 Immunoglobulin A, Qn, Serum mg/dL 2458-8
001685 Immunofixation, Serum 001792 Immunoglobulin M, Qn, Serum mg/dL 2472-9

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The LOINC® codes are copyright © 1994-2017, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf