Immunofixation (IFE) and Protein Electrophoresis, Random Urine

CPT: 84156; 84166; 86335
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Test Details


  • Bence Jones Protein Identification, Random Urine
  • Globulins, Random Urine
  • Myeloma Proteins, Random Urine
  • Urine Immunofixation for Myeloma Proteins

Test Includes

α1-Globulin, urine; α2-globulin, urine; β-globulin, urine; γ-globulin, urine; M-spike percentage; protein immunofixation, urine


Characterize the species of protein or proteins in cases of proteinuria. Techniques can identify albumin, γ-globulin, γ-globulin fragments (light chains, Bence Jones proteins); of value in the study of monoclonal gammopathies, lymphoproliferative diseases, including lymphoma; special application to multiple myeloma, macroglobulinemia of Waldenström, and amyloidosis; work up monoclonal gammopathy (M-spike)


Urine immunofixation (IFE) on agarose gel; urine protein electrophoresis on agarose media; visual light-chain typing

Specimen Requirements


Urine (random) (For 24-hour urine specimens, order test 003467.)


30-mL aliquot of random urine

Minimum Volume

11-mL aliquot of random urine


12-mL plastic transport tube (LabCorp N° 23597) without preservative


Collect first morning urine; mix well.

Storage Instructions


Causes for Rejection

Preservative added

Clinical Information

Special Instructions

The request form should include patient's age and clinical diagnosis.


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
122390 IFE and PE, Random Urine 013664 Protein,Total,Urine mg/dL 2888-6
122390 IFE and PE, Random Urine 133751 Albumin, U % 13986-5
122390 IFE and PE, Random Urine 133769 Alpha-1-Globulin, U % 13984-0
122390 IFE and PE, Random Urine 133777 Alpha-2-Globulin, U % 13987-3
122390 IFE and PE, Random Urine 133785 Beta Globulin, U % 13994-9
122390 IFE and PE, Random Urine 133793 Gamma Globulin, U % 13989-9
122390 IFE and PE, Random Urine 133794 M-Spike, % % 42484-6
122390 IFE and PE, Random Urine 013482 Immunofixation Result, Urine 13440-3
122390 IFE and PE, Random Urine 149533 Note: N/A
122390 IFE and PE, Random Urine 003724 PDF 11502-2

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