Immunofixation (IFE) and Protein Electrophoresis, Random Urine

CPT: 84156; 84166; 86335
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Synonyms

  • Bence Jones Protein Identification, Random Urine
  • Globulins, Random Urine
  • Myeloma Proteins, Random Urine
  • Urine Immunofixation for Myeloma Proteins

Test Includes

α1-Globulin, urine; α2-globulin, urine; β-globulin, urine; γ-globulin, urine; M-spike percentage; protein immunofixation, urine


Special Instructions

The request form should include patient's age and clinical diagnosis.


Expected Turnaround Time

3 - 5 days


Related Documents


Specimen Requirements


Specimen

Urine (random) (For 24-hour urine specimens, order test 003467.)


Volume

30-mL aliquot of random urine


Minimum Volume

11-mL aliquot of random urine


Container

12-mL plastic transport tube (Labcorp No. 23597) without preservative


Collection

Collect first morning urine; mix well.


Storage Instructions

Refrigerated (preferred) or room temperature


Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x1


Causes for Rejection

Preservative added


Test Details


Use

Characterize the species of protein or proteins in cases of proteinuria. Techniques can identify albumin, γ-globulin, γ-globulin fragments (light chains, Bence Jones proteins); of value in the study of monoclonal gammopathies, lymphoproliferative diseases, including lymphoma; special application to multiple myeloma, macroglobulinemia of Waldenström, and amyloidosis; work up monoclonal gammopathy (M-spike)


Methodology

Urine immunofixation (IFE) on agarose gel; urine protein electrophoresis on agarose media; visual light-chain typing


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
122390 IFE and PE, Random Urine 013664 Protein,Total,Urine mg/dL 2888-6
122390 IFE and PE, Random Urine 133751 Albumin, U % 13986-5
122390 IFE and PE, Random Urine 133769 Alpha-1-Globulin, U % 13984-0
122390 IFE and PE, Random Urine 133777 Alpha-2-Globulin, U % 13987-3
122390 IFE and PE, Random Urine 133785 Beta Globulin, U % 13994-9
122390 IFE and PE, Random Urine 133793 Gamma Globulin, U % 13989-9
122390 IFE and PE, Random Urine 133794 M-Spike, % % 42484-6
122390 IFE and PE, Random Urine 013482 Immunofixation Result, Urine 13440-3
122390 IFE and PE, Random Urine 149533 Note: N/A
122390 IFE and PE, Random Urine 003724 PDF 11502-2

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