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17-Hydroxypregnenolone, Pre- and Post-ACTH Stimulation
- 17-Hydroxypregnenolone (Two Specimens)
- Pregnenolone, 17-Hydroxy (Two Specimens)
This test may involve significant risk and should be performed only by qualified personnel who are familiar with the test and who have taken adequate precautions to protect the safety of the patient involved. Every effort has been made to ensure accuracy in these recommendations, but clinicians must use their judgment and refer to specific pharmaceutical resources to determine appropriate drug dosages for their patients.
High-pressure liquid chromatography (HPLC) tandem mass spectrometry
Serum (preferred) or plasma, frozen
1 mL (each specimen)
0.4 mL (Note: This volume does not allow for repeat testing.)
Transfer separated serum or plasma to a plastic transport tube. Note: Label each tube with patient's name, collection time, and date. Submit specimens simultaneously on the same test request form. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Causes for Rejection
Non-EDTA/heparin plasma; recently administered isotopes
Draw blood for baseline 17-OH pregnenolone. Inject cosyntropin 250 μg IM or IV (If IV, dilute cosyntropin in 2-5 mL of sterile saline and inject over two minutes). Draw blood for stimulated 17-OH pregnenolone 60 minutes after. See the online Endocrine Appendix: ACTH Stimulation for expected values for various steroids at baseline and after ACTH stimulation.