17-Hydroxypregnenolone, Pre- and Post-ACTH Stimulation

CPT: 84143(x2)
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Test Details


  • 17-Hydroxypregnenolone (Two Specimens)
  • Pregnenolone, 17-Hydroxy (Two Specimens)


This test may involve significant risk and should be performed only by qualified personnel who are familiar with the test and who have taken adequate precautions to protect the safety of the patient involved. Every effort has been made to ensure accuracy in these recommendations, but clinicians must use their judgment and refer to specific pharmaceutical resources to determine appropriate drug dosages for their patients.


High-pressure liquid chromatography (HPLC) tandem mass spectrometry

Specimen Requirements


Serum (preferred) or plasma, frozen


1 mL (each specimen)

Minimum Volume

0.4 mL (Note: This volume does not allow for repeat testing.)


Red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or green-top (heparin) tube


Transfer separated serum or plasma to a plastic transport tube. Note: Label each tube with patient's name, collection time, and date. Submit specimens simultaneously on the same test request form. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions


Stability Requirements




1 day


2 years

Causes for Rejection

Non-EDTA/heparin plasma; recently administered isotopes

Clinical Information

Special Instructions

Draw blood for baseline 17-OH pregnenolone. Inject cosyntropin 250 μg IM or IV (If IV, dilute cosyntropin in 2-5 mL of sterile saline and inject over two minutes). Draw blood for stimulated 17-OH pregnenolone 60 minutes after. See the online Endocrine Appendix: ACTH Stimulation for expected values for various steroids at baseline and after ACTH stimulation.


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
500770 17-OH Pregnenolone, ACTH Stim. 500771 17-OH Pregnenolone Baseline ng/dL 6765-2
500770 17-OH Pregnenolone, ACTH Stim. 500772 17-OH Pregnenolone Stimulated ng/dL 13604-4
500770 17-OH Pregnenolone, ACTH Stim. 501118 17-OH Pregnenolone, ACTH Stim. N/A
500770 17-OH Pregnenolone, ACTH Stim. 501977 17-OH Pregnenolone, ACTH Base N/A

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