17-Hydroxypregnenolone, Pre- and Post-ACTH Stimulation

CPT: 84143(x2)
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Test Details

Synonyms

  • 17-Hydroxypregnenolone (Two Specimens)
  • Pregnenolone, 17-Hydroxy (Two Specimens)

Use

This test may involve significant risk and should be performed only by qualified personnel who are familiar with the test and who have taken adequate precautions to protect the safety of the patient involved. Every effort has been made to ensure accuracy in these recommendations, but clinicians must use their judgment and refer to specific pharmaceutical resources to determine appropriate drug dosages for their patients.

Methodology

Radioimmunoassay (RIA)

Specimen Requirements

Specimen

Serum (preferred) or plasma, frozen

Volume

1 mL (each specimen)

Minimum Volume

0.4 mL (Note: This volume does not allow for repeat testing.)

Container

Red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or green-top (heparin) tube

Collection

Transfer separated serum or plasma to a plastic transport tube. Note: Label each tube with patient's name, collection time, and date. Submit specimens simultaneously on the same test request form. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Freeze

Stability Requirements

Temperature

Period

Refrigerated

1 day

Frozen

2 years

Causes for Rejection

Non-EDTA/heparin plasma; recently administered isotopes

Clinical Information

Special Instructions

Draw blood for baseline 17-OH pregnenolone. Inject cosyntropin 250 μg IM or IV (If IV, dilute cosyntropin in 2-5 mL of sterile saline and inject over two minutes). Draw blood for stimulated 17-OH pregnenolone 60 minutes after. See the online Endocrine Appendix: ACTH Stimulation for expected values for various steroids at baseline and after ACTH stimulation.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
500770 17-OH Pregnenolone, ACTH Stim. 500771 17-OH Pregnenolone Baseline ng/dL 6765-2
500770 17-OH Pregnenolone, ACTH Stim. 500772 17-OH Pregnenolone Stimulated ng/dL 13604-4
500770 17-OH Pregnenolone, ACTH Stim. 500780 17-OH Pregnenolone/ACTH Stim. N/A
500770 17-OH Pregnenolone, ACTH Stim. 501977 17-OH Pregnenolone, ACTH Base N/A
500770 17-OH Pregnenolone, ACTH Stim. 501118 17-OH Pregnenolone, ACTH Stim. N/A

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The LOINC® codes are copyright © 1994-2017, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf