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17-Hydroxypregnenolone, Mass Spectrometry (Endocrine Sciences)
High-pressure liquid chromatography/tandem mass spectrometry (LC/MS-MS)
• Premature (26 to 28 weeks): day 4: 375−3559 ng/dL
• Premature (31 to 35 weeks): day 4: 64−2380 ng/dL
• 3 days: 10−829 ng/dL
• 1 to 5 months: 36−763 ng/dL
• 6 to 11 months: 42−540 ng/dL
• 12 to 23 months: 14−207 ng/dL
• 24 months to 5 years: 10−103 ng/dL
• 6 to 9 years: 10−186 ng/dL
• Pubertal: 44−235 ng/dL
• Adults: 53−357 ng/dL
In humans, the adrenal glands and the gonads produce steroid hormones.1,2 The formation of pregnenolone from cholesterol is the first step in steroidogenesis. Steroidogenesis continues along two paths from pregnenolone. 17-Hydroxypregnenolone is produced from pregnenolone through the enzymatic action of 17-α-hydroxylase (17α-H). 3-β-hydroxysteroid dehydrogenase (3β-HSD) mediates the conversion of 17-hydroxypregnenolone to 17-hydroxyprogesterone. Alternatively, the enzyme 17,20 lyase can convert 17-hydroxypregnenolone to dehydroepiandrosterone (DHEA). 17α-H and 17,20 lyase activity are both mediated by a single microsomal cytochrome P450 complex.
17-hydroxypregnenelone levels have been shown to remain in the normal range in patients with Cushing.3 Levels can be suppressed with dexamethasone inhibition and increased with exogenous ACTH stimulation.3 Levels during the follicular phase of the menstrual cycle tend to be higher than during the luteal phase.3 17-hydroxypregnenelone levels have been shown to be elevated in patients with idiopathic hirsutism.4
Serum or plasma. frozen
0.3 mL adult; 0.1 mL pediatric (Note: This volume does not allow for repeat testing.)
Transfer the serum or plasma into a LabCorp PP transpak frozen purple tube with screw cap (LabCorp N° 49482) and freeze. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Causes for Rejection
Nonserum or non-EDTA plasma specimen received
|Order Code||Order Code Name||Order Loinc||Result Code||Result Code Name||UofM||Result LOINC|
|140715||17-Hydroxypregnenolone, MS||140725||17 OH Pregnenolone, Serum, MS||ng/dL||6765-2|
|140715||17-Hydroxypregnenolone, MS||500915||17 Hydroxypregnenolone||N/A|