Herpes Simplex Virus (HSV) Types 1/2, Amniotic Fluid, DNA PCR

CPT: 87529(x2)
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Test Details


  • HSV Types 1 and 2 DNA PCR (Real Time)


This test is intended for use as an aid to the diagnosis of herpes simplex virus (HSV) infections; also differentiates HSV-1 from HSV-2.


Real-time polymerase chain reaction (PCR)

Specimen Requirements


Amniotic fluid (noncentrifuged)


0.5 mL

Minimum Volume

0.2 mL


Sterile container


To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Freeze (preferred) or refrigerate.

Stability Requirements



Room temperature

7 days


7 days


90 days

Causes for Rejection

Quantity not sufficient for analysis; gross specimen contamination; specimen too old; leaking or broken tube

Clinical Information

Special Instructions

HSV PCR is a single test that both detects the presence of HSV DNA and determines which type is present in the positive samples. There is no mechanism, therefore, for testing for HSV-1 without simultaneously testing for HSV-2.


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
138594 HSV 1/2 PCR, Amniotic 139030 HSV 1/2 PCR-Amniotic Fluid N/A
138594 HSV 1/2 PCR, Amniotic 139335 HSV-1 DNA 44478-6
138594 HSV 1/2 PCR, Amniotic 139336 HSV-2 DNA 44503-1

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