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Herpes Simplex Virus (HSV) Culture and Typing
- ELVIS® HSV
- Five- to Seven-day Herpesvirus Culture/Type
- HSV Culture and Typing
- Viral Culture, HSV
- Virus Isolation, Herpes Simplex
Cell culture by ELVIS® method; identification and typing of herpes simplex virus. CPT coding for microbiology and virology procedures often cannot be determined before the culture is performed. Requests with only a written order and no test number indicated will be processed according to Default Testing for Virology.
Enzyme-linked virus-inducible system (ELVIS®). ELVIS® HSV was developed using a transgenic baby hamster kidney-21 cell line. In this cell line, HSV infection causes the expression of a beta-galactosidase “reporter gene”, and it is the expression of this gene that can be used to monitor infection by HSV. Cultures positive by ELVIS® are confirmed by immunofluorescent staining.
Infectious HSV is rarely present in CSF during encephalitis, resulting in a poor recovery by culture (<5% in adults, <50% in children). Studies have shown a >98% detection of HSV DNA in CSF by polymerase chain reaction (PCR), and this method is now considered the standard for diagnosis of HSV encephalitis. For HSV PCR testing, please request test 138651. HSV is occasionally isolated from the CSF of patients with HSV-2 meningitis and of neonates with congenital herpes, and from urine of patients with primary genital HSV infections concurrent with cystitis.
Vesicular fluid, ulcerated lesions, pharyngeal and throat swabs, urine, cerebrospinal fluid (CSF), autopsy and biopsy material, eye exudates, vaginal swabs
Swab in viral transport media, 1 mL fluid, 0.5 g tissue in transport medium
Specimen is best collected within the first three days after appearance of lesion but no more than seven days. After collection, remove cap from the transport media tube and insert swab aseptically into transport tube and break swab shaft. Replace cap so that swab will not interfere with closure and allow tube to leak. Label tube with patient identification and transport to the laboratory at 4°C.
Causes for Rejection
Bacterial swab specimen; specimen received in grossly leaking transport container; dry specimen; specimen submitted in fixative or additive; specimen received in expired transport media or incorrect transport device; inappropriate specimen transport conditions; specimen stored or transported greater than seven days refrigerated; specimen stored or transported at room temperature; wooden shaft swab in transport device; unlabeled specimen or name discrepancy between specimen and test request label
Submit one specimen per test requested. Specify the exact specimen source/origin (eg, genital lesion). Indicate a specific test number on the request form. Check expiration date of transport; do not use expired devices.
|Order Code||Order Code Name||Order Loinc||Result Code||Result Code Name||UofM||Result LOINC|
|008250||HSV Culture and Typing||5859-4||057943||HSV Culture/Type||5859-4|