Hepatitis C Virus (HCV), Quantitative, RNA PCR (Nongraphical) With Reflex to Genotyping

CPT: 87522
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Test Details

Synonyms

  • HCV Real-time PCR With Reflex to Genotype
  • HCV RNA Quantitation With Reflex to Genotype

Use

Quantitation of hepatitis C virus with reflex to HCV genotype if viral load is >1000 IU/mL

Limitations

The quantifiable range of the assay is 15 IU/mL to 100,000,000 IU/mL.

Methodology

Cobas® AmpliPrep/Cobas® TaqMan® HCV Test v 2.0. See Hepatitis C Virus (HCV) Genotyping, Nonreflex [550475] for more information.

Specimen Requirements

Specimen

Serum or plasma

Volume

3 mL

Minimum Volume

1 mL (Note: This volume does not allow for repeat testing.)

Container

Gel-barrier tube, lavender-top (EDTA) tube, or plasma preparation tube (PPT™)

Collection

Centrifuge sample within 24 hours of collection. Transfer serum/plasma to a screw-cap polypropylene transport tube. Ship frozen (preferred). Plasma from a PPT™ can be frozen and shipped in situ. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit a separate frozen specimen for each test requested.

Storage Instructions

Freeze (preferred) or refrigerate. Stable at room temperature for 24 hours or refrigerated for 72 hours. Stable frozen for six weeks.

Causes for Rejection

Incorrect anticoagulant; PPT™ not centrifuged

Clinical Information

Special Instructions

This procedure does not provide serial monitoring; it is intended for one-time use only. If serial monitoring is required, please order test 550100.

References

Kleiber J, Walter T, Haberhausen G, Tsang S, Babiel R, Rosenstraus M. Performance characteristics of a quantitative, homogeneous TaqMan RT-PCR test for HCV RNA. J Mol Diagn. 2000 Aug; 2(3):158-166. 11229521

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