Hemoglobin (Hb) Solubility With Reflex to Hemoglobinopathy Fractionation Cascade, Blood

CPT: 85660. If reflex testing is performed, concomitant CPT codes/charges will apply.
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Synonyms

  • Hb Solubility
  • Sickle Cell Preparation
  • Sickle Cell Solubility Test
  • Sickle Cell Test
  • Sickle Prep
  • Sickledex™

Test Includes

Hemoglobin (Hb) solubility and automatic reflex to hemoglobin (Hb) fractionation blood (at additional charge), if positive.


Expected Turnaround Time

2 - 3 days


Related Information


Related Documents


Specimen Requirements


Specimen

Whole blood


Volume

1 mL


Minimum Volume

0.6 mL (Note: This volume does not allow for repeat testing.)


Container

Lavender-top (EDTA) tube; capillary puncture: three microhematocrit tubes

Note: Because this profile reflexes to Hemoglobinopathy Fractionation Cascade [121690] when positive, only a Lavender-top (EDTA) tube is allowed.


Collection

To avoid delays in turnaround time, please submit a separate lavender-top tube for each test requiring a lavender-top.


Storage Instructions

Room temperature


Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3


Patient Preparation

No preliminary fluid or food restriction is required.


Causes for Rejection

Specimen other than whole blood received; clotted specimen


Test Details


Use

Qualitative determination of presence of hemoglobin S; detect sickling hemoglobins; evaluate hemolytic anemia, undiagnosed hereditary anemia with morphologic (sickle-like) abnormalities on peripheral blood smear



Methodology

Sodium hydrosulfite reduction


Reference Interval

Negative


Additional Information


References

Chehab FF, Kan YW. Detection of sickle cell anaemia mutation by colour DNA amplification. Lancet. 1990 Jan 6; 335(8680):15-17. 1967329
Miale JB. Laboratory Medicine: Hematology. 6th ed. St Louis, Mo: Mosby-Year Book Inc;1982:624-625.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
005330 HB Solu + Rflx Frac 6864-3 005224 Hemoglobin (Hgb) Solubility 6864-3
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121692 Hgb F % 32682-7
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121693 Hgb A % 20572-4
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121694 Hgb A2 % 4552-6
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121695 Hgb S % 32683-5
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex 2 121697 Hgb Solubility 121698 Hgb Solubility 6864-3
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex 2 121697 Hgb Solubility 121719 Final Interpretation: 12710-0
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex 2 121710 Hgb Fractionation by HPLC 121712 Hgb F % 42246-9
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex 2 121710 Hgb Fractionation by HPLC 121713 Hgb A % 42244-4
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex 2 121710 Hgb Fractionation by HPLC 121714 Hgb A2 % 42245-1
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex 2 121710 Hgb Fractionation by HPLC 121715 Hgb S % 44923-1
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex 2 121710 Hgb Fractionation by HPLC 121716 Hgb C % 44920-7
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex 2 121710 Hgb Fractionation by HPLC 121717 Hgb E % 44922-3
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex 2 121710 Hgb Fractionation by HPLC 121718 Hgb Variant % 42248-5
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex 2 121710 Hgb Fractionation by HPLC 121719 Final Interpretation: 12710-0
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex 2 121720 Hgb Frac by HPLC+Solubility 121698 Hgb Solubility 6864-3
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex 2 121720 Hgb Frac by HPLC+Solubility 121712 Hgb F % 42246-9
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex 2 121720 Hgb Frac by HPLC+Solubility 121713 Hgb A % 42244-4
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex 2 121720 Hgb Frac by HPLC+Solubility 121714 Hgb A2 % 42245-1
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex 2 121720 Hgb Frac by HPLC+Solubility 121715 Hgb S % 44923-1
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex 2 121720 Hgb Frac by HPLC+Solubility 121716 Hgb C % 44920-7
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex 2 121720 Hgb Frac by HPLC+Solubility 121717 Hgb E % 44922-3
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex 2 121720 Hgb Frac by HPLC+Solubility 121718 Hgb Variant % 42248-5
Reflex Table for Hemoglobin (Hgb) Solubility
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121690 Hgb Fractionation Cascade 121696 Interpretation: 12710-0
Reflex 2 121720 Hgb Frac by HPLC+Solubility 121719 Final Interpretation: 12710-0

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