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Evaluate anemia, blood loss, hydration or suspected polycythemic condition, and response to treatment
Hyperlipemic plasma (especially Fredrickson and Lees type I and V in which chylomicronemia is present) or white count >50,000/mm³ may falsely elevate the hemoglobin result with corresponding increase in the MCH. The laboratory will attempt to correct this interference by a special technique.
Automated cell counter
Tube fill capacity
0.5 mL (500 μL for pediatric microtainer capillary tubes; fill tube to capacity.)
Lavender-top (EDTA) tube
Invert tube 8 to 10 times immediately after tube is filled at the time of collection.
Maintain specimen at room temperature.
Causes for Rejection
Hemolysis; clotted specimen; tube not filled with minimum volume; improper labeling; transport tubes with whole blood; specimen received with plasma removed; specimen diluted or contaminated with IV fluid; specimen drawn in any anticoagulant other than EDTA
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