Hemoglobin (Hb)

CPT: 85018
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Expected Turnaround Time

Within 1 day


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Specimen Requirements


Specimen

Whole blood


Volume

Tube fill capacity


Minimum Volume

0.5 mL (500 μL for pediatric microtainer capillary tubes; fill tube to capacity.)


Container

Lavender-top (EDTA) tube


Collection

Invert tube 8 to 10 times immediately after tube is filled at the time of collection.


Storage Instructions

Maintain specimen at room temperature.


Stability Requirements

Temperature

Period

Room temperature

1 day

Refrigerated

3 days

Frozen

Unstable

Freeze/thaw cycles

Unstable


Causes for Rejection

Hemolysis; clotted specimen; tube not filled with minimum volume; improper labeling; transport tubes with whole blood; specimen received with plasma removed; specimen diluted or contaminated with IV fluid; specimen drawn in any anticoagulant other than EDTA


Test Details


Use

Evaluate anemia, blood loss, hydration or suspected polycythemic condition, and response to treatment


Limitations

Hyperlipemic plasma (especially Fredrickson and Lees type I and V in which chylomicronemia is present) or white count >50,000/mm³ may falsely elevate the hemoglobin result with corresponding increase in the MCH. The laboratory will attempt to correct this interference by a special technique.


Methodology

Automated cell counter


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