Giardia lamblia, Direct Detection EIA and Cryptosporidium, Direct Detection EIA

CPT: 87328; 87329
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Test Details

Synonyms

  • Cryptosporidium Antigen

Test Includes

Cryptosporidium antigen; Giardia lamblia antigen

Use

Rapid detection of Giardia and Cryptosporidium from clinical samples.

Methodology

Enzyme immunoassay (EIA)

Specimen Requirements

Specimen

Stool

Volume

2 Grams (thumbnail size portion of stool) or 2 mL liquid stool

Minimum Volume

2 Grams or 2 mL

Container

O&P transport vial with formalin (Para-Pak® pink); stool C&S transport vial with Cary Blair medium (Para-Pak® orange); sterile screw cap container or Para-Pak® white

Collection

Fecal specimens for parasitic examination should be collected before initiation of antidiarrheal therapy or antiparasitic therapy. The highest yield on hospitalized patients occurs when diarrhea is present on admission or within 72 hours of admission. The onset of diarrhea more than 72 hours after admission is usually caused by Clostridium difficile toxin rather than parasites or the usual stool pathogens. The following recommendations are made for efficient and cost-effective diagnosis of diarrheal disease in patients admitted with gastroenteritis.

• Submit one or two specimens per diarrheal illness immediately. Consider first requesting EIAs for Giardia and Cryptosporidium (See test Giardia lamblia, Direct Detection EIA [182204] or Giardia lamblia, Direct Detection EIA and Cryptosporidium, Direct Detection EIA [183558]). Giardia and Cryptosporidium are the most common causes of parasitic gastroenteritis in the United States.

• If EIAs are negative, request add-on testing for Ova and Parasites Examination [008623], and submit an additional specimen after five days for O&P examination.

• Also consider testing for unusual stool pathogens (Cyclospora Smear, Stool [183145]), especially in the immunocompromised patient.

Storage Instructions

Maintain stool specimens in O&P transport with formalin at room temperature or refrigerate and test within two months of collection. Stool collected in Cary Blair medium should be refrigerated or frozen and tested within one week of collection. Stool in sterile container should be frozen or can be kept refrigerated if tested within 48 hours.

Causes for Rejection

Inappropriate specimen transport device; specimens in PVA; specimens other than stool; specimens received other than described; inadequate labeling

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
183558 Giardia/Cryptosporidium EIA 182204 Giardia lamblia Ag, EIA 6412-1
183558 Giardia/Cryptosporidium EIA 183021 Cryptosporidium EIA 6371-9

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The LOINC® codes are copyright © 1994-2017, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf