Test Details
Result Turnaround Time
1 - 4 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
This test is used to determine the presence or absence of fungal forms in clinical specimens submitted for direct microscopic exam.
Custom Additional Information
The fungal stain is performed using KOH/Calcofluor. Calcofluor white is a fluorescent dye that binds nonspecifically to chitin in the fungal (yeast and mold) cell wall. To improve visualization under a fluorescence microscope, potassium hydroxide (KOH) is added to help clear thick or viscous samples.
Specimen Requirements
Specimen
Biopsy, body fluid, aspirates, bronchoalveolar lavage (BAL), skin, nails, hair, sinus, sputum, swab of conjunctiva, throat, tissue, urine or vaginal
Volume
For optimal recovery: 2 mL or 1 cm3 tissue, 10 mL blood, whole nails, 5-10 mL body fluids and BAL, 3-5 mL CSF, 5 mL aspirates or sputum
Minimum Volume
1 mL body fluid including BAL and CSF; aspirates or sputum
Container
Sterile leakproof container or swab
Collection Instructions
Biopsy: Surgical specimen in sterile container. A small amount of sterile nonbacteriostatic water should be used to prevent drying.
Body fluid, aspirates: Aspirated material in sterile container.
Skin and nails: Cleanse the area with 70% alcohol prior to specimen collection. Nail scraping should be from a subsurface portion of the infected nail. Skin should be taken from the active border of the lesion.
Hair: Epilate 10 to 12 hairs and place them in a sterile container.
Swabs of throat, nose, nasopharynx, ear, vagina: Swab affected area or visible lesion.
Urine: Clean catch midstream sample in sterile container.
Wound: Purulent material, fluid, scraping of lesion border, or swab (least preferred) in sterile transport container. Swabs cannot be split for other tests.
Avoid contamination of the specimen with commensal organism as much as possible. Specify the source of the specimen and include any pertinent clinical information.
Storage Instructions
Maintain specimen at room temperature.
Patient Preparation
Usual sterile preparation (see Blood Culture, Routine [008300]).
Causes for Rejection
Fungal stain (KOH/Calcofluor) cannot be performed on blood; unlabeled specimen or name discrepancy between specimen and request label; specimen submitted in syringe with needle attached; specimen received after prolonged transport (usually more than 72 hours; lithium heparin tube; stool; swab without evidence of specimen present; specimen received after leaking transport container into specimen bag (Trach-suction devices will often leak if the cap with tubing is not removed and replaced by solid cap. If tubing cannot be replaced by a solid cap, the specimen should be transferred to a leakproof sterile cup with a screwcap); inappropriate transport device
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 008136 | Fungus Stain | 21003-9 | 008136 | Fungus Stain | 21003-9 | |
| Order Code | 008136 | |||||
| Order Code Name | Fungus Stain | |||||
| Order Loinc | 21003-9 | |||||
| Result Code | 008136 | |||||
| Result Code Name | Fungus Stain | |||||
| UofM | ||||||
| Result LOINC | 21003-9 |
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 080086 | Result | 080087 | Result 1 | N/A | |
| Reflex 1 | ||||||
| Order Code | 080086 | |||||
| Order Name | Result | |||||
| Result Code | 080087 | |||||
| Result Name | Result 1 | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 080086 | Result | 080088 | Result 2 | N/A | |
| Reflex 1 | ||||||
| Order Code | 080086 | |||||
| Order Name | Result | |||||
| Result Code | 080088 | |||||
| Result Name | Result 2 | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 080086 | Result | 080089 | Result 3 | N/A | |
| Reflex 1 | ||||||
| Order Code | 080086 | |||||
| Order Name | Result | |||||
| Result Code | 080089 | |||||
| Result Name | Result 3 | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 080086 | Result | 080090 | Result 4 | N/A | |
| Reflex 1 | ||||||
| Order Code | 080086 | |||||
| Order Name | Result | |||||
| Result Code | 080090 | |||||
| Result Name | Result 4 | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 080086 | Result | 080091 | Antimicrobial Susceptibility | N/A | |
| Reflex 1 | ||||||
| Order Code | 080086 | |||||
| Order Name | Result | |||||
| Result Code | 080091 | |||||
| Result Name | Antimicrobial Susceptibility | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||