Epstein-Barr Virus (EBV), DNA Probe

CPT: 88365
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Test Details

Synonyms

  • EBV in situ Hybridization

Test Includes

DNA probe hybridization for EBV in formalin-fixed, paraffin-embedded tissue

Use

Detect EBV DNA sequences in tissue sections; adjunct to histopathology

Limitations

Failure to detect target DNA may result from several factors, eg, improper sampling, handling, and/or fixative of the specimen, or target DNA below the sensitivity limits of the assay; therefore, lack of staining does not necessarily imply the absence of target DNA.

This test was developed, and its performance characteristics determined, by LabCorp. It has not been cleared or approved by the US Food and Drug Administration (FDA). The FDA has determined that such clearance or approval is not necessary.

Methodology

In situ hybridization

Specimen Requirements

Specimen

Formalin-fixed, paraffin-embedded (FFPE) tissue biopsy. Tissue biopsies should be fixed in 10% neutral-buffered formalin and embedded in paraffin within 72 hours. Do not submit frozen tissue or substitute other fixatives. For tissues submitted in formalin, please provide the appropriate gross/microscopic description indicating the number, type, and sites of the tissue submitted.

Container

Submit paraffin block in crushproof container. Submit fresh tissue in a sterile container in 10% neutral-buffered formalin.

Collection

Tissue biopsies should be fixed in 10% neutral buffered formalin and embedded in paraffin within 72 hours. Do not submit frozen tissue or substitute other fixatives. For tissues submitted in formalin, please provide appropriate gross/microscopic description indicating the number, type, and site of the tissue(s) submitted.

Storage Instructions

Maintain specimen at room temperature.

Causes for Rejection

Insufficient tissue; improper fixation; inappropriate fixative

Clinical Information

Special Instructions

Please direct any questions regarding this test to oncology customer service at 800-345-4363.

References

Berg LC, Copenhaver CM, Morrison VA, et al. B-cell lymphoproliferative disorders in solid-organ transplant patients: Detection of Epstein-Barr virus by in situ hybridization. Hum Pathol. 1992 Feb; 23(2):159-163. 1310951
De Souza YG, Freese UK, Greenspan D, Greenspan JS. Diagnosis of Epstein-Barr virus infection in hairy leukoplakia by using nucleic acid hybridization and noninvasive techniques. J Clin Microbiol. 1990 Dec; 28(12):2775-2778. 2177752
Hamilton-Dutoit SJ, Delecluse HJ, Raphael M, Lenoir G, Pallesen G. Detection of Epstein-Barr virus genomes in AIDS related lymphomas: sensitivity and specificity of in situ hybridisation compared with Southern blotting. J Clin Pathol. 1991 Aug; 44(8):676-680. 1653789

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
550913 Epstein-Barr Virus, DNA Probe 5004-7 550919 EBV, DNA Probe 5004-7

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