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Dihydrotestosterone (DHT) (Endocrine Sciences)
Dihydrotestosterone and dihydrotestosterone/ratio may be used for differential diagnosis of diseases of sexual differentiation (DSD). DHT levels may be inappropriately low compared to testosterone levels in 5a-reductase deficiency. It is recommended by guidelines to measure the ratio of testosterone to DHT.
DHT levels may also be monitored to follow the effectiveness of 5a-reductase inhibitor treatment in female alopecia.
High-pressure liquid chromatography (HPLC) with tandem mass spectrometry
Serum (preferred) or plasma, frozen
0.5 mL (Note: This volume does not allow for repeat testing.)
Red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or green-top (heparin) tube
Separate serum or plasma from cells within one hour of collection. Send serum or plasma in plastic transport tube. Transfer specimen to a plastic transport tube before freezing. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
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