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Test Details
Synonyms
- 11-Desoxycortisol
- Compound S for Metyrapone Test
Use
Evaluate hypothalamic-pituitary-adrenal axis and pituitary ACTH reserve
Methodology
Liquid chromatography/tandem mass spectrometry (LC/MS-MS) after chromatography and solvent extraction
Additional Information
11-Deoxycortisol is the immediate precursor of cortisol and follows the same catabolic pathways as cortisol. The conversion of 11-deoxycortisol to cortisol is inhibited by metyrapone, which acts on 11-β-hydroxylase. The metyrapone test (see the online Endocrine Appendix: ACTH Stimulation) serves as a reliable and sensitive indicator of pituitary ACTH secretory reserve. The 11-deoxycortisol levels normally increase to 100 times the control value following metyrapone administration. Reduced response occurs in patients with hypoadrenalism or with hypopituitarism and in some patients with diseases of the hypothalamus. Patients with myxedema, some pregnant patients, and those on oral contraceptives respond poorly.
Specimen Requirements
Specimen
Serum, frozen
Volume
0.5 mL
Minimum Volume
0.2 mL (Note: This volume does not allow for repeat testing.)
Container
Red-top tube or gel-barrier tube
Patient Preparation
No isotopes administered 24 hours prior to venipuncture
Collection
Transfer the serum into a LabCorp PP transpak frozen purple tube with screw cap (LabCorp N° 49482). Freeze immediately and maintain frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Storage Instructions
Freeze.
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 1 day |
Refrigerated | 1 day |
Frozen | 2 years |
Freeze/thaw cycles | Stable x3 |
Causes for Rejection
Recently administered isotopes
Clinical Information
References
Sindler BH, Griffing GT, Melby JC, et al. The superiority of the metyrapone test versus the high-dose dexamethasone test in the differential diagnosis of Cushing's syndrome. Am J Med. 1983 Apr; 74(4):657-662. 6837591
Spark RF. Simplified assessment of pituitary-adrenal reserve: Measurement of serum 11-deoxycortisol after metyrapone. Ann Intern Med. 1971 Nov; 75(5):717-723.4330677
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 500550 | 11-Desoxycortisol | 500551 | Desoxycortisol(Compound S),S | ug/dL | 1657-6 |