Cyclic AMP, Plasma

CPT: 82030
Updated on 11/3/2017
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Test Details

Synonyms

  • cAMP, Plasma

Use

Determination of cAMP is useful in the clinical assessment of parathyroid function.

Methodology

Radioimmunoassay (RIA)

Additional Information

Cyclic adenosine-3'-5'-monophosphate (cAMP) serves as a second messenger in signal transduction of various hormones, like adrenaline, ACTH, LH, FSH, glucagon, and calcitonin. The hormone itself is the first messenger, binding to specific membrane receptors of hormone-responsive cells. This leads to the activation of an enzyme located at the inner side of the cell membrane, called adenylate cyclase, which catalyzes the synthesis of cAMP from ATP. The increased cellular level of cAMP initiates the activation of protein kinases. The determination of cAMP in urine or plasma has become more and more important in the clinical assessment of parathyroid functions. The parathyroid hormone (PTH) stimulates the synthesis of cAMP in the renal cortex and an increased secretion of cAMP. Cyclic AMP is a specific marker of circulating PTH: 90% of patients with hyperparathyroidism show increased levels of nephrogenic cAMP. The determination of cAMP is also significant for the differential diagnosis of hypercalcemia

Specimen Requirements

Specimen

Plasma, frozen

Volume

0.5 mL

Minimum Volume

0.2 mL (Note: this volume does not allow for repeat testing.)

Container

Lavender-top (EDTA) tube

Patient Preparation

Patient should have no radioactive isotopes administered within 48 hours prior to venipuncture.

Collection

Keep blood samples on ice and centrifuge as soon as possible. Separate plasma from cells and transfer to a plastic transport tube. Freeze immediately. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Freeze.

Stability Requirements

Temperature

Period

Frozen

9 days

Freeze/thaw cycles

Stable x3

Temperature

Period

Room temperature

7 days

Refrigerated

7 days

Frozen

9 days

Freeze/thaw cycles

Stable x3

Temperature

Period

Frozen

9 days

Freeze/thaw cycles

Stable x3

Frozen

9 days

Freeze/thaw cycles

Stable x3

Causes for Rejection

Gross hemolysis; gross lipemia; nonfrozen specimen received; non-EDTA plasma specimen received

Clinical Information

References

Buchs N, Bonjour JP, Rizzoli R. Renal tubal reabsorption of phosphate is positively related to the extent of bone metastatic load in patients with prostate cancer. J Clin Endocrinol Metab. 1998 May; 83(5):1535-1541. 9589651
Chaudhary LR, Avioli LV. Identification of mitogen-activated protein (MAP) kinase in normal human osteoblastic and bone marrow stomal cells: Attenuation of MAP kinase activation by cAMP, parathyroid hormone, and forskolin. Mol Cell Biochem. 1998 Jan; 178(1-2):59-68. 9546582
Hagiwara H, Hiruma Y, Inoue A, Yamaguchi A, Hirose S. Deceleration by angiotensin II of the differentiation and bone formation of rat calvarial osteoblastic cells. J Endocrinol. 1998 Mar; 156(3):543-550. 9582511
Hamacher C, Studer H, Zbaeren J, Schatz H, Derwahl M. Expressions of functional stimulatory guanine nucleotide binding protein in nonfunctioning thyroid adenomas is not correlated to adenylate cyclase activity and growth of these tumors. J Clin Endocrinol Metab. 1995 May; 80(5):1724-1732. 7745026
Hilal G, Martel-Pelletier J, Pelletier JP, Ranger P, Lajeunesse D. Osteoblast-like cells from human subchondral osteoarthritic bone demonstrate an altered phenotype in vitro: Possible role in subchondral bone sclerosis. Arthritis Rheum. 1998 May; 41(5):891-899. 9588742
Horton JK, Martin RC, Kalinka S, et al. Enzyme immunoassays for the estimation of adenosine 3',5' cyclic monophosphate and guanosine 3',5' cyclic monophosphate in biological fluids. J Immunol Methods. 1992 Oct 19; 155(1):31-40. 1328396
Pradelles P, Grassi J, Chabardes D, Guiso N. Enzyme immunoassays of adenosine cyclic 3',5'-monophosphate and guanosine cyclic 3',5'-monophosphate using acetylcholinesterase. Anal Chem. 1989 Mar 1; 61(5):447-453. 2541635
Riminucci M, Fisher LW, Shenker A, Spiegel AM, Bianco P, Gehron Robey P. Fibrous dysplasia of bone in the McCune-Albright syndrome: Abnormalities in bone formation. Am J Pathol. 1997 Dec; 151(6):1587-1600. 9403710
Shane E, Parisien M, Henderson JE, et al. Tumor-induced osteomalacia: Clinical and basic studies. J Bone Miner Res. 1997 Sep; 12(9):1502-1511. 9286768
Tintut Y, Parhami F, Boström K, Jackson SM, Demer LL. cAMP stimulates osteoblast-like differentiation of calcifying vascular cells. Potential signaling pathway for vascular calcification. J Biol Chem. 1998 Mar 27; 273(13):7547-7553. 9516456
Tsai JA, Bucht E, Stark A, Sjöstedt U, Törring O. Parathyroid hormone-related protein (1-37) induces cAMP response in human osteoblast-like cells. Calcif Tissue Int. 1998 Mar; 62(3):250-254. 9501959
Wilson NJ, Jaworowski A, Ward AC, Hamilton JA. cAMP enhances CSF-1-induced ERK activity and c-fos mRNA expression via MEK-dependent and Ras-independent mechanism in macrophages. Biochem Biophys Res Commun. 1998 Mar 17; 244(2):475-480. 9514945

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
004984 Cyclic AMP, Plasma 25498-7 004985 Cyclic AMP, Plasma pmol/mL 25498-7

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