Cryptosporidium, Direct Detection EIA

CPT: 87328
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Expected Turnaround Time

3 - 5 days


Related Documents

For more information, please view the literature below.

Cryptosporidium spp and Giardia lamblia


Specimen Requirements


Specimen

Stool


Volume

2 g (thumbnail size portion of stool) or 2 mL liquid stool


Minimum Volume

2 g or 2 mL


Container

O&P transport vial with formalin (Para-Pak® pink); stool C&S transport vial with Cary Blair medium (Para-Pak® orange); sterile screw-cap container or Para-Pak® white


Collection

Fecal specimens for parasitic examination should be collected before initiation of antidiarrheal therapy or antiparasitic therapy. The highest yield on hospitalized patients occurs when diarrhea is present on admission or within 72 hours of admission. The onset of diarrhea more than 72 hours after admission is usually caused by Clostridium difficile toxin rather than parasites or the usual stool pathogens. The following recommendations are made for efficient and cost-effective diagnosis of diarrheal disease in patients admitted with gastroenteritis.

• Submit one or two specimens per diarrheal illness immediately. Consider first requesting EIAs for Giardia and Cryptosporidium (See test Giardia lamblia, Direct Detection EIA [182204] or Giardia lamblia, Direct Detection EIA and Cryptosporidium, Direct Detection EIA [183558]). Giardia and Cryptosporidium are the most common causes of parasitic gastroenteritis in the United States.

• If EIAs are negative, request add-on testing for Ova and Parasites Examination [008623], and submit an additional specimen after five days for O&P examination.

• Also consider testing for unusual stool pathogens (Cyclospora Smear, Stool [183145]), especially in the immunocompromised patient.


Storage Instructions

Maintain stool specimens in O&P transport with formalin at room temperature or refrigerate and test within 18 months of collection. Stool collected in Cary Blair transport should be refrigerated or frozen at -20°C or less until tested. Stool in sterile container or clean vial transport should be kept between 2-8°C and tested within 24 hours of collection or frozen at -20°C or less until tested.


Causes for Rejection

Inappropriate specimen transport device; specimens in PVA; specimens other than stool; specimens received other than described; inadequate labeling


Test Details


Use

Rapid detection of Cryptosporidium from clinical samples.


Methodology

Enzyme immunoassay (EIA)


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
183020 Cryptosporidium EIA 6371-9 183021 Cryptosporidium EIA 6371-9

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