Coenzyme Q10, Total

CPT: 82542
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Test Details

Synonyms

  • CoQ10
  • Ubiquinone 50

Methodology

High-pressure liquid chromatography (HPLC) with electrochemical detection

Reference Interval

0.37−2.20 μg/mL

Additional Information

Coenzyme Q10 (CoQ10; Ubiquinone 50) is an endogenous compound found in most human tissues. It possesses multiple cellular and extracellular functions, primarily based on it ability to undergo redox cycling. Such redox cycling plays an important role in vivo. Examples include CoQ10's involvement in ATP synthesis (as part of the electron transport chain of the inner membrane of the mitochondrion) and its ability to act as an antioxidant in the prevention of lipid peroxidation.

Specimen Requirements

Specimen

Plasma, frozen and protected from light

Volume

1 mL

Minimum Volume

0.5 mL

Container

Green-top (heparin) tube, amber plastic transport tube with amber-top. (If amber tubes are unavailable, cover standard transport completely, top and bottom, with aluminum foil. Identify specimen with patient name directly on the container and on the outside of the aluminum foil. Secure with tape.) For amber plastic transport tube and amber-top, order LabCorp N° 23598.

Collection

Draw blood into chilled green-top tube. Gently invert five to six times to mix with preservatives. Keep unopened vacuum tube on ice before processing. Immediately separate plasma from red cells by centrifugation. Plasma must be separated from cells within 45 minutes of venipuncture. Transfer plasma specimen to a labeled amber transport tube and freeze on dry ice. Protect from light. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Freeze plasma in plastic amber transport tube with amber-top on dry ice. Protect from light. (Where dry ice is not available at the collection site, the sample may be kept refrigerated or frozen at -20°C for three days, at which time it must be transferred to dry ice for storage. Samples must be shipped to the laboratory on dry ice.)

Stability Requirements

Temperature

Period

Refrigerated

3 days

Frozen

14 days at -70°C

Freeze/thaw cycles

Stable x3

Causes for Rejection

Use of EDTA anticoagulant;1 specimen not protected from light; plasma not frozen within 24 hours of collection

Clinical Information

Footnotes

1. Tang PH, Miles MV, Steele P, et al. Anticoagulant effects on plasma coenzyme Q10 estimated by HPLC with coulometric detection. Clin Chim Acta. 2002 Apr; 318(1-2):127-131. 11880122

References

Tang PH, Miles MV, DeGrauw A, Hershey A, Pesce A. HPLC analysis of reduced and oxidized coenzyme Q10 in human plasma. Clin Chem. 2001 Feb; 47(2):256-265. 11159774
Tang PH, Miles MV, Miles L, et al. Measurement of reduced and oxidized coenzyme Q9 and coenzyme Q10 levels in mouse tissues by HPLC with coulometric detection. Clin Chim Acta. 2004 Mar; 341(1-2):173-184. 14967174

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
120251 Coenzyme Q10, Total 27923-2 120252 Coenzyme Q10, Total ug/mL 27923-2

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