CD4:CD8 Ratio Profile

CPT: 86360
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Test Details

Test Includes

Percentage CD4+; absolute CD4+; percentage CD8+; absolute CD8+; CD4:CD8 ratio; CBC with differential

Methodology

Flow cytometry

Reference Interval

Reference intervals for adults have been established by the laboratory. See table.

Reference Intervals for Lymphocyte Immunophenotyping

CD

Age

95% Confidence Interval

Percentage

Cells/mm3

Minimum

Maximum

Minimum

Maximum

Pediatric reference intervals are from Comans-Bitter WM, de Groot R, van den Beemd R, et al. Immunophenotyping of blood lymphocytes in childhood. Reference values for lymphocyte subpopulations. J Pediatrics. 1997 Mar; 130(3):388-393.

CD4+

Neonates

17

52

400

3500

1 wk to 2 m

41

68

1700

5300

2 to 5 m

33

58

1500

5000

5 to 9 m

33

58

1400

5100

9 to 15 m

31

54

1000

4600

15 to 24 m

25

50

900

5500

2 to 5 y

23

48

500

2400

5 to 10 y

27

53

300

2000

10 to 16 y

25

48

400

2100

Adults

31

59

359

1519

CD8+

Neonates

10

41

200

1900

1 wk to 2 m

9

23

400

1700

2 to 5 m

11

25

500

1600

5 to 9 m

13

26

600

2200

9 to 15 m

12

28

400

2100

15 to 24 m

11

32

400

2300

2 to 5 y

14

33

300

1600

5 to 10 y

19

34

300

1800

10 to 16 y

9

35

200

1200

Adults

12

36

109

897

T4:T8 ratio

Adults

0.92

3.72

Additional Information

HIV-1 infection results in a decrease of CD4 T cells, an increase of CD8 T cells, a decrease in the CD4:CD8 ratio, and a progressive destruction of immune function. In HIV-1 seropositive patients, enumeration of CD4 T cells may be used for prognostic purposes and to monitor disease progression and antiretroviral therapy.

Specimen Requirements

Specimen

Whole blood

Volume

Fill tube(s) to capacity.

Container

Lavender-top (EDTA) tube and yellow-top (ACD-A) or (ACD-B) tube

Collection

Invert tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimen so it will arrive in the laboratory within 48 hours of collection. Indicate date and time of venipuncture on the tube(s) and on the test request form.

Storage Instructions

Maintain specimen at room temperature.

Stability Requirements

Temperature

Period

Room temperature

2 days

Refrigerated

Unstable

Frozen

Unstable

Freeze/thaw cycles

Unstable

Causes for Rejection

Hemolysis; specimen refrigerated or frozen; clotted specimen; contaminated specimen

Clinical Information

References

Giorgi J, Kesson A, Chou CC. Immunodeficiency and infectious diseases. In: Rose N, deMacario E, Fahey J, et al, eds. Manual of Clinical Laboratory Immunology. 4th ed. Washington, DC: ASM Press;1992:chap 25.
Stevens RA, Lempicki RA, Natarajan V, et al. General immunologic evaluation of patients with immunodeficiency virus infection. In: Detrick B, Hamilton RG, Folds JD, et al, eds. Manual of Molecular and Clinical Laboratory Immunology. 7th ed. Washington, DC: ASM Press;2006:chap 95.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
505271 CD4/CD8 Ratio Profile 505010 Absolute CD 4 Helper /uL 24467-3
505271 CD4/CD8 Ratio Profile 505009 % CD 4 Pos. Lymph. % 8123-2
505271 CD4/CD8 Ratio Profile 505579 Abs. CD 8 Suppressor /uL 14135-8
505271 CD4/CD8 Ratio Profile 505580 % CD 8 Pos. Lymph. % 8101-8
505271 CD4/CD8 Ratio Profile 505273 CD4/CD8 Ratio 54218-3
505271 CD4/CD8 Ratio Profile 005025 WBC x10E3/uL 6690-2
505271 CD4/CD8 Ratio Profile 005033 RBC x10E6/uL 789-8
505271 CD4/CD8 Ratio Profile 005041 Hemoglobin g/dL 718-7
505271 CD4/CD8 Ratio Profile 005058 Hematocrit % 4544-3
505271 CD4/CD8 Ratio Profile 015065 MCV fL 787-2
505271 CD4/CD8 Ratio Profile 015073 MCH pg 785-6
505271 CD4/CD8 Ratio Profile 015081 MCHC g/dL 786-4
505271 CD4/CD8 Ratio Profile 105007 RDW % 788-0
505271 CD4/CD8 Ratio Profile 015172 Platelets x10E3/uL 777-3
505271 CD4/CD8 Ratio Profile 015107 Neutrophils % 770-8
505271 CD4/CD8 Ratio Profile 015123 Lymphs % 736-9
505271 CD4/CD8 Ratio Profile 015131 Monocytes % 5905-5
505271 CD4/CD8 Ratio Profile 015149 Eos % 713-8
505271 CD4/CD8 Ratio Profile 015156 Basos % 706-2
505271 CD4/CD8 Ratio Profile 115398 Immature Cells N/A
505271 CD4/CD8 Ratio Profile 015909 Neutrophils (Absolute) x10E3/uL 751-8
505271 CD4/CD8 Ratio Profile 015917 Lymphs (Absolute) x10E3/uL 731-0
505271 CD4/CD8 Ratio Profile 015925 Monocytes(Absolute) x10E3/uL 742-7
505271 CD4/CD8 Ratio Profile 015933 Eos (Absolute) x10E3/uL 711-2
505271 CD4/CD8 Ratio Profile 015941 Baso (Absolute) x10E3/uL 704-7
505271 CD4/CD8 Ratio Profile 015108 Immature Granulocytes % 38518-7
505271 CD4/CD8 Ratio Profile 015911 Immature Grans (Abs) x10E3/uL 51584-1
505271 CD4/CD8 Ratio Profile 015945 NRBC % 58413-6
505271 CD4/CD8 Ratio Profile 015180 Hematology Comments: 18314-5
505271 CD4/CD8 Ratio Profile 000000 HIV Confidential N/A
Reflex Table for Immature Granulocytes
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 015111 Please note: 015111 Please note: N/A
Reflex Table for Immature Grans (Abs)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 015111 Please note: 015111 Please note: N/A
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115247 Neutrophils % 23761-0
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115250 Lymphs % 737-7
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115251 Monocytes % 744-3
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115252 Eos % 714-6
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115253 Basos % 707-0
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115398 Immature Cells N/A
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115254 Neutrophils Absolute X10E3/uL 753-4
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115255 Lymphs (Absolute) X10E3/uL 732-8
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115256 Monocytes(Absolute) X10E3/uL 743-5
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115257 Eos (Absolute Value) X10E3/uL 712-0
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115258 Baso(Absolute) X10E3/uL 705-4
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 115260 Differential Comment 18723-7
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 005034 RBC Comment 6742-1
Reflex Table for Hematology Comments:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115246 Differential 015171 Platelet Comment 9317-9

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