Bordetella pertussis and Bordetella parapertussis, Real-time DNA PCR

CPT: 87798(x2)
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Test Details

Synonyms

  • B pertussis and B parapertussis, DNA PCR (Real-time)
  • Whooping Cough

Use

This assay is intended to be used as an aid to the diagnosis of Bordetella pertussis (whooping cough) and Bordetella parapertussis (whooping cough-like syndrome).

Limitations

The target for the B pertussis PCR reaction, a region of IS481, is also found in Bordetella holmesii. A false-positive result for B pertussis DNA may occur if B holmesii is present in the sample tested.

Methodology

Real-time polymerase chain reaction (PCR)

Specimen Requirements

Specimen

Nasopharyngeal (NP) swab, throat swab (dacron or rayon), or nasopharyngeal aspirate/wash

Volume

One NP/throat swab or 0.5 mL nasal aspirate/wash

Container

• Option 1 (preferred): Flocked NP swab submitted dry in plastic transport sleeve (PeopleSoft N° 93307)• Option 2 (alternate): NP swab submitted in charcoal-containing transport media• Option 3 (alternate): NP/throat swab submitted in universal transport media (UTM)• Option 4 (alternate): NP wash/aspirate submitted in sterile container

Storage Instructions

Maintain specimen at room temperature or refrigerate. Do not freeze charcoal transport swabs or dry swabs. Swabs in UTM and NP aspirates/washes may be frozen for seven days.

Stability Requirements

Temperature

Period

Room temperature

5 days

Refrigerated

7 days

Causes for Rejection

Calcium alginate swabs; swabs in noncharcoal-containing transport media; swabs in Regan-Lowe culture media

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
138677 B.pertussisB.parapertussis PCR 138679 Bordetella pertussis DNA 23826-1
138677 B.pertussisB.parapertussis PCR 138680 Bordetella parapertussis DNA 29723-4

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The LOINC® codes are copyright © 1994-2017, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf