Bordetella pertussis and Bordetella parapertussis, Real-time DNA PCR

CPT: 87798(x2)
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Test Details


  • B pertussis and B parapertussis, DNA PCR (Real-time)
  • Whooping Cough


This assay is intended to be used as an aid to the diagnosis of Bordetella pertussis (whooping cough) and Bordetella parapertussis (whooping cough-like syndrome).


The target for the B pertussis PCR reaction, a region of IS481, is also found in Bordetella holmesii. A false-positive result for B pertussis DNA may occur if B holmesii is present in the sample tested.


Real-time polymerase chain reaction (PCR)

Specimen Requirements


Nasopharyngeal (NP) swab, throat swab (dacron or rayon), or nasopharyngeal aspirate/wash


One NP/throat swab or 0.5 mL nasal aspirate/wash


• Option 1 (preferred): Flocked NP swab submitted dry in plastic transport sleeve (PeopleSoft N° 93307)

• Option 2 (alternate): NP swab submitted in charcoal-containing transport media

• Option 3 (alternate): NP/throat swab submitted in universal transport media (UTM)

• Option 4 (alternate): NP wash/aspirate submitted in sterile container

Storage Instructions

Maintain specimen at room temperature or refrigerate. Do not freeze charcoal transport swabs or dry swabs. Swabs in UTM and NP aspirates/washes may be frozen for seven days.

Stability Requirements



Room temperature

5 days


7 days

Causes for Rejection

Calcium alginate swabs; swabs in noncharcoal-containing transport media; swabs in Regan-Lowe culture media


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
138677 B.pertussisB.parapertussis PCR 138679 Bordetella pertussis DNA 23826-1
138677 B.pertussisB.parapertussis PCR 138680 Bordetella parapertussis DNA 29723-4

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