Adult Acute Lymphoblastic Leukemia (ALL) Profile, FISH

CPT: Call client services. 88271(x8); 88275(x4)
Updated on 10/10/2019
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Synonyms

  • Acute Lymphoblastic Leukemia; Leukemia/Lymphoma-specific FISH

Test Includes

Fluorescence in situ hybridization (FISH) for acute lymphoblastic leukemia (ALL), targeting rearrangement of BCR/ABL, KMT2A (MLL), CMYC and numerical changes of chromosome 6 and 21q deletion

Fluorescence in situ hybridization (FISH) for acute lymphoblastic leukemia (ALL), targeting rearrangement of BCR/ABL, TCF3, KMT2A (MLL), ETV6/RUNX1, and numerical changes of 4, 10, 17, and CDKN2A deletion

Fluorescence in situ hybridization (FISH) for acute lymphoblastic leukemia (ALL), targeting rearrangement of BCR/ABL, KMT2A (MLL), CMYC and numerical changes of chromosome 6 and 21q deletion


Special Instructions

Include the pertinent clinical diagnosis and previous cytogenetic studies with the test request form.


Expected Turnaround Time

3 - 5 days



Related Documents


Specimen Requirements


Specimen

Blood, bone marrow, CSF, fixed-cell pellet from a cytogenetic analysis, slide with metaphase and/or interphase nuclei or bone marrow touch preparation slides are acceptable for testing.


Volume

10 mL blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 8 slides with metaphase and/or interphase nuclei or 8 touch preparation slides.


Minimum Volume

5 mL blood (pediatric), 1 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 4 slides with metaphase and/or interphase nuclei or 4 touch preparation slides. (Note: Minimum volumes may not allow for repeat testing.)


Container

Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube (suboptimal). Adjust tube size to sample volume to avoid heparin toxicity.


Collection

Transport to the testing laboratory at room temperature, use of a cool pack or a LabCorp transport kit is acceptable. Do not allow samples to overheat or freeze.


Storage Instructions

Maintain the specimen at either room temperature or refrigerate. Do not freeze.


Causes for Rejection

Broken Vacutainer®; broken or stained slides; paraffin blocks; paraffin slides; decalcified bone cores; frozen specimen; quantity not sufficient for analysis


Test Details


Use

Diagnostic and prognostic test for acute lymphoblastic leukemia in the pediatric population; detection rate is improved from 50% with a chromosome study to 90% with fluorescence in situ hybridization (FISH)


Methodology

Fluorescence in situ hybridization (FISH)


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
511077 FISH Adult ALL Oncology 511059 Specimen Type 31208-2
511077 FISH Adult ALL Oncology 511061 Cells Counted 62361-1
511077 FISH Adult ALL Oncology 511068 Cells Analyzed 62360-3
511077 FISH Adult ALL Oncology 511069 FISH Result 62356-1
511077 FISH Adult ALL Oncology 511073 Interpretation 62365-2
511077 FISH Adult ALL Oncology 511076 Director Review: 48672-0
511077 FISH Adult ALL Oncology 512123 PDF 80563-0

For Providers

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The LOINC® codes are copyright © 1994-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf