Adult Acute Lymphoblastic Leukemia (ALL) Profile, FISH

CPT: 88271(x8); 88275(x4)
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Test Details

Synonyms

  • Acute Lymphoblastic Leukemia; Leukemia/Lymphoma-specific FISH

Test Includes

Fluorescence in situ hybridization (FISH) for acute lymphoblastic leukemia (ALL), targeting rearrangement of BCR/ABL, TCF3, KMT2A (MLL), ETV6/RUNX1, and numerical changes of 4, 10, 17, and CDKN2A deletion

Use

Diagnostic and prognostic test for acute lymphoblastic leukemia in the pediatric population; detection rate is improved from 50% with a chromosome study to 90% with fluorescence in situ hybridization (FISH)

Methodology

Fluorescence in situ hybridization (FISH). FISH probes include translocation probes for BCR/ABL1, KMT2A (MLL), MYC, and enumeration probes for chromosome 6 and 21q (RUNX1).

Specimen Requirements

Specimen

Bone marrow, blood (with excess blast), cerebrospinal fluid (CSF), bone marrow touch prep slides, fixed-cell pellet from a cytogenetic analysis or slides prepared from a cytogenetic analysis

Volume

3 mL bone marrow (For additional cytogenetic studies an additional 1 mL of bone marrow is required.); 10 mL blood; one tube fixed-cell pellet; or ten slides

Minimum Volume

5 mL blood, 1 mL bone marrow (For additional cytogenetic studies, an additional 1 mL of bone marrow is required.); fixed-cell pellet from a cytogenetic analysis or four slides

Container

Green-top (sodium heparin) tube (pediatric Vacutainer® is optimal) or lavender-top (EDTA) tube

Storage Instructions

Maintain specimens at room temperature.

Causes for Rejection

Broken or dirty slides; excessive cellular debris or stained slides; broken Vacutainer®; frozen specimen

Clinical Information

Special Instructions

Include the pertinent clinical diagnosis and previous cytogenetic studies with the test request form.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
511077 FISH Adult ALL Oncology 511059 Specimen Type 31208-2
511077 FISH Adult ALL Oncology 511061 Cells Counted 62361-1
511077 FISH Adult ALL Oncology 511068 Cells Analyzed 62360-3
511077 FISH Adult ALL Oncology 511069 FISH Result 62356-1
511077 FISH Adult ALL Oncology 511073 Interpretation 62365-2
511077 FISH Adult ALL Oncology 511076 Director Review: 48672-0

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CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2017, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf