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Anti-Nuclear Antibodies by Indirect Fluorescent Antibody (IFA), Cerebrospinal Fluid (RDL)

CPT: 86038

Expected Turnaround Time

7 - 14 days

Specimen Requirements


Cerebrospinal fluid


2 mL

Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)


Sterile transport tube


Collect cerebrospinal fluid in sterile transport tube.

Storage Instructions

Refrigerate or freeze.

Stability Requirements



Room temperature

7 days


14 days


60 days

Freeze/thaw cycles

Stable x1

Causes for Rejection

Grossly hemolyzed, bacterial contamination, lipemic, icteric, incorrect specimen types

Test Details


ANA are commonly found in a variety of autoimmune diseases. Antibody frequency increases with age in apparently healthy people. ANA patterns on HEp-2 slides provide only general clues about particles (chromatin, nucleosomes, and spliceosomes). ANA patterns (other than centromere pattern) are not reliably correlated with the presence of specific antibodies and must be further evaluated by EIA using individual ENA antigens.


This test was developed and its performance characteristics determined by LabCorp. It has not been cleared or approved by the Food and Drug Administration.


Indirect Fluorescent Antibody (IFA)

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