Virus, Direct Detection DFA, Respiratory Syncytial Virus (RSV)

CPT: 87280
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Synonyms

  • Respiratory Syncytial Virus, Direct Detection by DFA

Test Includes

Direct microscopic examination (nonculture) of virus-infected cells. Quality of specimen may be evaluated.


Special Instructions

Submit one specimen per test requested. Specify the exact specimen source/origin (eg, nasopharynx). Indicate a specific test number on the request form.


Expected Turnaround Time

1 - 4 days



Related Documents


Specimen Requirements


Specimen

Impression smears of tissues, lesion scrapings and swabs, upper respiratory tract swabs


Volume

Two slides per virus


Container

Plain glass slide, no fixative


Collection

Submit two thinly made air-dried slides. Do not use spray fixative.


Storage Instructions

Refrigerate or maintain specimen at room temperature.


Causes for Rejection

Specimen received after prolonged delay in transport (usually more than 72 hours); unlabeled specimen or name discrepancy between specimen and request label; broken or badly scratched slides; fixative used on slides; bacterial swab transport


Test Details


Use

Rapid diagnosis of RSV


Methodology

Direct fluorescent antibody (DFA)


Reference Interval

No virus detected


Additional Information

Generally, this test is not as sensitive as cell culture.


References

Drew WL. Controversies in viral diagnosis. Rev Infect Dis. 1986 Sep-Oct; 8(5):814-824 (review). 3024292
Drew WL. Diagnostic virology. Clin Lab Med. 1987 Dec; 7(4):721-740 (review). 3319366
Smith TF. Rapid methods for the diagnosis of viral infections. Lab Med. 1987; 18:16-20.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
185017 Virus, RSV Ag, DFA 5877-6 185017 Virus, RSV Ag, DFA 5877-6

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