Antiphosphatidylserine, IgA, IgG, IgM

CPT: 86148(x3)
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Expected Turnaround Time

2 - 4 days



Related Documents

For more information, please view the literature below.

Procedures for Hemostasis and Thrombosis: A Clinical Test Compendium


Specimen Requirements


Specimen

Serum


Volume

1 mL


Minimum Volume

0.5 mL


Container

Red-top tube or gel-barrier tube


Collection

Separate serum from cells by centrifugation after clot formation.


Storage Instructions

Refrigerate


Causes for Rejection

Gross hemolysis; bacterial contamination; lipemia


Test Details


Use

An adjunct to β2-glycoprotein and anticardiolipins in diagnosing aPS (antiphospholipid syndrome) for assessing thrombotic risk and other obstetric complications such as miscarriage or fetal growth restriction


Methodology

Enzyme-linked immunosorbent assay (ELISA)


Additional Information

Antiphospholipid antibodies are a heterogeneous group of immunoglobulins that bind to several anionic phospholipids, including cardiolipin and phosphatidylserine. High serum levels of antiphospholipid antibodies are frequently detected in patients with autoimmune (ie, SLE) and nonautoimmune diseases, as well as in apparently healthy individuals. These antibodies have been associated with an increased risk for recurrent arterial and venous thrombotic events, thrombocytopenia, and fetal loss. These manifestations are the main features of the antiphospholipid syndrome. Antiphospholipid antibodies are detected either by ELISAs using cardiolipin or other negatively-charged phospholipids as the antigen (anticardiolipin antibodies) or coagulation assays (lupus anticoagulants). Unlike cardiolipin, phosphatidylserine is a more physiologically relevant phospholipid due to its presence in cell membranes of endothelial cells and platelets and its role in the coagulation cascade. The detection of antiphosphatidylserine (aPS) antibodies by ELISA has been recommended for the serological diagnosis of antiphospholipid syndrome. Patients with positive reactions to both cardiolipin and phosphatidylserine are more likely to have clinical complications than those positive for only one. Higher prevalence and mean serum levels of antiphosphatidylserine antibodies have been reported in autoimmune patients. In addition, antiphosphatidylserine antibodies in SLE patients correlated with clinical manifestations of antiphospholipid syndrome and their pathogenic role has been demonstrated in a murine model.


References

Barna LK, Triplett DA, Foster WM, et al. A study of relationships among antiphosphatidylserine and anticardiolipin antibodies, the lupus anticoagulant and clinical implications. Lupus. 1994; 3:357.
Corgenix Inc. The significance of IgA antiphosphatidylserine (aPS) antibodies. The Reader. 2000; 10(2). Antiphosphatidylserine Semiquantitative Test Kits [package insert ].
Keedy KJ, Buckner TR, Taylor DO, et al. Antiphospholipid syndrome: Detection and clinical significance of antiphosphatidylserine antibodies by ELISA. 49th Annual AACC Clinical Laboratory Meeting, July 22nd-24th, 1997.
Keedy KJ, Santos M, Lopez L. Antiphosphatidylserine antibodies require beta 2-glycoprotein 1 as cofactor in ELISA. Lupus. 1994: 3:327.
Keedy KJ, Santos M, Lopez L. Prevalence and clinical significance of antiphosphatidylserine antibodies. Arthritis Rheum. 1994; 37:S392.
Miyakis S, Lockshin MD, Atsumi T, et al. International consensus statement on an update of the classification criteria for definite antiphospholipid syndrome (APS). J Thromb Haemost. 2006 Feb; 4(2):295-306. 16420554
Yodfat O, Blank M, Krause I, Schoenfeld Y. The pathogenic role of antiphosphatidylserine antibodies: Active immunization with the antibodies leads to the induction of antiphospholipid syndrome. Clin Immunol Immunopathol. 1996 Jan; 78(1):14-20. 8599879
Zuazu-Jausoro I, Oliver A, Monserrat I, et al. Importance of antiphosphatidylserine antibodies in patients with lupus anticoagulant. Analysis of 30 cases. Med Clin (Barc). 1990 Jul 7; 95(6):210-213. 2123507

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
117994 Antiphosphatidylserine IgG/M/A 117926 Antiphosphatidylserine IgA APS IgA 13069-0
117994 Antiphosphatidylserine IgG/M/A 117927 Antiphosphatidylserine IgG GPS IgG 14245-5
117994 Antiphosphatidylserine IgG/M/A 117910 Antiphosphatidylserine IgM MPS IgM 14246-3

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