Hepatitis C Virus (HCV), RNA PCR, Qualitative
| Hepatitis C Virus (HCV), RNA PCR, Qualitative | | | |
| Number | | 550400 |
| CPT | | 87521 |
| Synonyms | | HCV RNA, PCR, Qualitative |
| Test Includes | | Detection of HCV |
| Special Instructions | | Submit a separate request form for each frozen specimen. |
| Specimen | | Serum or plasma, frozen |
| Volume | | 1 mL |
| Minimum Volume | | 0.5 mL |
| Container | | Plasma preparation tube (PPT™) or screw-cap polypropylene frozen transport tube |
| Collection | | Collect specimen in gel-barrier tube, PPT™, yellow-top (ACD) tube, or lavender-top (EDTA) tube. Do not use green-top (heparin) tubes. Centrifuge specimen with 6 hours of collection. Remove serum/plasma, transfer specimen to polypropylene screw-cap tube (not “pop top” or “snap cap”), and freeze. Specimens drawn into PPT™ need to be centrifuged within 6 hours and frozen. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested. |
| Storage Instructions | | Freeze |
| Causes for Rejection | | Hemolysis; green-top (heparin) tube; specimen not frozen; PPT™ not centrifuged; specimen received in “pop-top” or “snap-cap” tube |
| Use | | Detect and confirm hepatitis C virus infection |
| Methodology | | Polymerase chain reaction (PCR) amplification and DNA probe detection |
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