Hepatitis C Virus (HCV), Quantitative, RNA (Abbott® RealTime)

CPT: 87522
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  • Viral Load, Abbott, Real-time

Expected Turnaround Time

3 - 7 days

Related Documents

Specimen Requirements


Serum or plasma


3.5 mL

Minimum Volume

1.1 mL (Note: This volume does not allow for repeat testing.)


PPT™, gel-barrier tube, or lavender-top (EDTA) tube


Serum or plasma (EDTA) specimens may be used. Freshly drawn specimens (whole blood) may be held at 2°C to 30°C for six hours. Centrifuge specimen within six hours of collection. Remove serum or plasma, transfer to a screw-cap polypropylene transport tube. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Freeze. Stable at room temperature for six hours (whole blood) or one day after centrifugation. Stable refrigerated for 24 hours (whole blood) or three days after centrifugation. Freeze for long-term storage.

Causes for Rejection

Hemolysis; heparinized plasma; nonfrozen specimens; specimen received in "pop-top" or "snap-cap" tube; whole blood

Test Details


Determine the number of international units (IU) of hepatitis C virus (HCV) RNA per milliliter of serum or plasma in known HCV-positive patients


Assay range: 12 IU/mL to 100,000,000 IU/mL


Abbott m2000® real-time HCV assay


Huang S, Salituro J, Tang N, et al. Thermodynamically modulated partially double-stranded linear DNA probe design for homogeneous real-time PCR. Nucleic Acids Res. 2007; 35(16):e101. 17693434
Tang N, Huang S, Salituro J, et al. A realtime HIV-1 viral load assay for automated quantitation of HIV-1 RNA in genetically diverse group M subtypes A-H, group O and group N samples. J Virol Methods. 2007 Dec; 146(1-2):236-245. 17707519
Vermehren J, Yu ML, Monto A, et al. Multi-center evaluation of the Abbott RealTime HCV assay for monitoring patients undergoing antiviral therapy for chronic hepatitis C. J Clin Virol. 2011 Oct; 52(2):133-137. 21803650


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
551300 HCV RealTime Abbott 11011-4 551301 Hepatitis C Quantitation IU/mL 11011-4
551300 HCV RealTime Abbott 11011-4 551302 HCV log10 log10 IU/mL 38180-6
551300 HCV RealTime Abbott 11011-4 551304 Test Information: N/A
Reflex Table for Hepatitis C Quantitation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551305 HCV RNA (International Units) 551306 HCV RNA (International Units) IU/mL 11011-4
Reflex Table for Hepatitis C Quantitation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551305 HCV RNA (International Units) 551307 HCV log10 log10 IU/mL 38180-6

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